Inhibitors of the JNK signal transduction pathway and methods of use

ABSTRACT

JNK-interacting protein 1 (JIP-1), an inhibitor of the JNK1 protein, and methods of treating a pathological condition or of preventing the occurrence of a pathological condition in a patient by the administration of a therapeutically effective amount of JIP-1 polypeptides, peptides, peptide mimetics, or nucleic acids are described.

RELATED APPLICATION INFORMATION

This application is a divisional of application Ser. No. 08/819,177,filed Apr. 28, 1997, now U.S. Pat. No. 6,043,083 which is incorporatedherein by reference in its entirety.

STATEMENT AS TO FEDERALLY SPONSORED RESEARCH

This invention was made, in part, with Government support under grantsCA58396 and CA65831 awarded by the National Cancer Institute. Thegovernment may have certain rights in the invention.

FIELD OF THE INVENTION

The invention relates to signal transduction inhibitors.

BACKGROUND OF THE INVENTION

The c-Jun NH2-terminal kinase (JNK) is a member of the stress-activatedgroup of Mitogen Activated Protein kinases (MAP kinases) implicated inthe control of cell growth. The JNK signal transduction pathway isactivated in response to environmental stress and by the engagement ofseveral classes of cell surface receptors, including cytokine receptors,serpentine receptors, and receptor tyrosine kinases. Whitmarsh et al.,J. Mol. Med., 74:589 (1996). In addition, genetic studies of Drosophilahave demonstrated that JNK is required for early embryonic development.Sluss et al., Genes & Dev., 10:2745 (1996); Riesgo-Escovar et al., Genes& Dev. 10:2759 (1996). In mammalian cells, JNK has been implicated inthe immune response, oncogenic transformation, and apoptosis. JNKmediates these-effects, at least in part, by increasing the expressionof target genes. Targets of the JNK signal transduction pathway includethe transcription factors c-Jun, ATF2, and Elk-1. Whitmarsh et al.,supra.

JNK is activated in the liver by metabolic oxidative stress. Mendelsonet al., Proc. Natl. Acad. Sci. USA 93:12908-12913 (1996). Activation ofJNK also occurs in the kidney during stress, for example, during Bchemicrenal failure. Demari et al., Am. J. Physiol. 272:F292-F298 (1997). JNKis also activated during cardiovascular disease such as ischemiaIreperfusion and during organ transplantation. Pombo et al., J. Biol.Chem. 269:26546-26551 (1994); Force et al., Circ. Res. 78:947-53 (1996).

While JNK is located in both the cytoplasmic and the nuclearcompartments of quiescent cells, activation of the JNK signaltransduction pathway is associated with accumulation of JNK in thenucleus. Mechanisms governing this sub-cellular distribution have notbeen previously elucidated.

Anchor or tethering proteins play an important role in the regulation ofmultiple signal transduction pathways. These anchor proteins, whichinclude the nuclear factor kappa B (NFkB) inhibitor IkB, the A kinaseanchor protein (AKAP) group of proteins that bind the type II cyclicadenosine monophosphate (AMP) dependent protein kinase, and the p190protein that binds Ca²⁺-calmodulin-dependent protein kinase II, localizetheir tethered partners to specific sub-cellular compartments. Verma etal., Genes & Dev., 9:2723 (1995); McNeill et al., J. Biol. Chem.,270:10043 (1995); Faux et al., Trends Biochem. Sci., 21:312 (1996)).Anchor proteins also target enzymes to specific substrates, and createmulti-enzyme signaling complexes, such as the Ste5 MAP kinase scaffoldcomplex and the AKAP79 kinase/phosphatase scaffold complex. Choi et al.,Cell, 78:499 (1994); Klauck et al., Science, 271:1589 (1996); Faux etal., Cell, 85:9 (1996)).

SUMMARY OF THE INVENTION

The invention, which is based on the discovery of a cytoplasmic anchorprotein, JNK-interacting protein 1 (JIP-1; SEQ ID NO:1), features JIP-1polypeptides and nucleic acids, therapeutic compositions containingthese polypeptides and nucleic acids, and methods of administering thesecompositions. JIP-1 specifically binds to and inhibits the biologicaleffects of JNK, including the initiation of apoptosis and oncogenictransformation. JIP-1 is therefore useful as a therapeutic agent fortreating pathological conditions characterized by apoptosis ortransformation. For example, JIP-1 compositions can be used to treatneurodegenerative diseases characterized by apoptosis, includingParkinson's disease and Alzheimer's disease; and blood clots, which leftuntreated could result in stroke and associated memory loss. Otherconditions that can be treated using the compositions and methods of theinvention are autoimmune diseases such as arthritis; other conditionscharacterized by inflammation; and malignancies, such as leukemias,e.g., chronic myelogenous leukemia (CML). other conditions that can betreated with JIP-1 compositions include oxidative damage to organs suchas the liver and kidney, and heart disease, particularly damage due toischemia/reperfusion and cardiomyopathy. JIP-1 compositions can also beused to treat donor organs for transplantation. These organs are exposedto substantial environmental stress, the effects of which are blocked byJNK inhibitors such as JIP-1.

The invention features a substantially pure JIP-1 polypeptide. A “JIP-1polypeptide” is a protein having an amino acid sequence thatspecifically binds JNK to the same extent, or at least 10% of thebinding activity of wildtype JIP-1. Such polypeptides can be from 5 to200 amino acids in length, e.g., from 10 to 100 amino acids in length,or from 20 to 50 amino acids in length. Such polypeptides include theJNK Binding Domain (JBD), or portions thereof, of JIP-1 (e.g., aminoacids 148 to 174, forming the “core” of the JBD of wildtype JIP-1, shownin FIG. 2, and having the sequence SGDTYRPKRPTTLNLFPQVPRSQDTLN; SEQ IDNO:3). JIP-1 polypeptides are preferably derived from a mammal, such asa mouse or a human.

In various embodiments, the polypeptide is soluble, the polypeptideincludes the JNK-binding domain of JIP-1 or a portion thereof, thepolypeptide is at least 80%, 90%, or 100% identical to the amino acidsequence from amino acid 148 to amino acid 174 of JIP-1 (the coreJNK-binding domain; SEQ ID NO:3), or the polypeptide has an amino acidsequence identical to the amino acid sequence from amino acid 148 toamino acid 174 of JIP-1 (SEQ ID NO:3), or the polypeptide is at least80%, 90%, or 100% identical to the amino acid sequence from amino acid127 to amino acid 281 of JIP-1 (the JNK-binding domain; SEQ ID NO:4).

The polypeptides of the invention can be modified to enhance theiruptake by cells. Such modifications increase the hydrophobicity ofmolecules to facilitate passage through the lipid bilayer of the cellmembrane. For example, polypeptides can be complexed with myristic acidor packaged in liposomes. Alternatively, JIP-1 polypeptides can becomplexed with hydrophobic moieties (e.g., lipids) or peptides thatincrease the delivery of proteins into cells.

The invention also includes peptide mimetics of JIP-1 polypeptides. A“peptide mimetic” of a known polypeptide is a compound that mimics theactivity of the peptide or polypeptide, but which is composed ofmolecules other than, or in addition to, amino acids.

By “polypeptide” is meant any chain of amino acids, regardless of lengthor post-translational modification (e.g., glycosylation orphosphorylation), and thus includes peptides, proteins, and fusionproteins.

A “substantially identical” polypeptide sequence differs from a givensequence only by conservative amino acid substitutions or by one or morenonconservative substitutions, deletions, or insertions located atpositions which do not destroy the function of the polypeptide comparedto wildtype JIP-1. Polypeptides of the invention can be 70%, 80%, 85%,90%, or 95% identical to wildtype JIP-1.

A “substantially pure” preparation is at least 60% by weight of thecompound of interest, e.g., a JIP-1 polypeptide or fragment of a JIP-1polypeptide. Preferably the preparation is at least 75%, more preferablyat least 90%, and more preferably at least 95% by weight of the compoundof interest. Purity can be measured by any appropriate standard method,e.g., column chromatography, polyacrylamide gel electrophoresis, or HighPressure Liquid Chromatography (HPLC) analysis.

The polypeptides of the invention include, but are not limited to,recombinant polypeptides, natural polypeptides, and syntheticpolypeptides, as well as preproteins or proproteins and biologicallyactive fragments. A “biologically active fragment” of JIP-1 is afragment having at least 50%, 70%, 80%, 90%, 95%, or 100% or greater, ofthe activity of naturally occurring or synthetic, full length JIP-1.

The polypeptides of the invention can be physically linked to anotherpolypeptide, e.g., a marker polypeptide. For example, the polypeptidecan be fused to a hexa-histidine tag to facilitate purification ofbacterially expressed proteins, or a hemagglutinin tag to facilitatepurification of protein expressed in eukaryotic cells.

In another aspect, the invention features an isolated nucleic acid thatincludes a sequence encoding a JIP-1 polypeptide or a fragment of such apolypeptide. Preferably, the nucleic acid is derived from a mammal.

The invention also encompasses nucleic acids that hybridize understringent conditions (as described herein) to a nucleic acid encoding aJIP-1 polypeptide. Stringent conditions include hybridization at 68° C.in 5×SSC/5×Denhardt's solution/1.0% SDS, or in 0.5 M NaHPO₄ (pH 7.2)/1mM EDTA/7% SDS, or in 50% formamide/0.25 M NaHPO₄ (pH 7.2)/0.25 M NaCl/1mM EDTA/7% SDS; and washing in 0.2×SSC/0.1% SDS at room temperature orat 42° C., or in 0.1×SSC/0.1% SDS at 68° C., or in 40 mM NaHPO₄ (pH7.2)/1 mM EDTA/5% SDS at 50° C., or in 40 mM NaHPO₄ (pH 7.2) 1 mMEDTA/1% SDS at 50° C. Moderately stringent conditions include washing in3×SSC at 42° C. The parameters of salt concentration and temperature canbe varied to achieve the desired level of identity between the probe andthe target nucleic acid. For guidance regarding such conditions see,e.g., Sambrook et al., Molecular Cloning, A Laboratory Manual, ColdSpring Harbor Press, Cold Spring Harbor, N.Y.; and Ausubel et al.,supra, at Unit 2.10.

The hybridizing portion of the hybridizing nucleic acid is preferably20, 30, 50, or 70 bases long. The hybridizing portion of the hybridizingnucleic acid can be 95% or even 98% or 100% identical to the sequence ofa portion of a nucleic acid encoding a JIP-1 polypeptide. Hybridizingnucleic acids of the type described above can be used as a cloningprobe, a primer (e.g., a PCR primer), or a diagnostic probe. Preferredhybridizing nucleic acids encode a polypeptide having some or all of thebiological activities possessed by naturally-occurring JIP-1. Thus, theymay encode a protein that is shorter or longer than the various forms ofJIP-1 described herein. Hybridizing nucleic acids can also encodeproteins that are related to JIP-1, e.g., proteins encoded by genes thatinclude a portion having a relatively high degree of identity to a JIP-1gene described herein.

The term “nucleic acid” encompasses both RNA and DNA, including cDNA,genomic DNA, and synthetic (e.g., chemically synthesized) DNA. Thenucleic acid may be double-stranded or single-stranded. Wheresingle-stranded, the nucleic acid may be the sense strand or theantisense strand.

An “isolated nucleic acid” is a nucleic acid that is free of the nucleicacids that normally flank it in the genome. The term therefore includes,e.g., a recombinant nucleic acid incorporated into a vector, such as anautonomously replicating plasmid or virus; a cDNA or genomic DNAfragment produced by polymerase chain reaction (PCR) or restrictionendonuclease treatment; and recombinant DNA which is part of a hybridgene encoding additional polypeptide sequences.

A “substantially identical” nucleic acid is a nucleic acid with asequence that is at least 50%, preferably 70%, and more preferably 85%,90%, or 95% homologous to a given nucleic acid sequence, e.g., SEQ IDNO:2.

The invention also features transformed cells harboring a nucleic acidencompassed by the invention. Vectors and plasmids that include anucleic acid properly positioned for expression are also within theinvention. A “transformed cell” is a cell into which (or into anancestor of which) has been introduced, by means of recombinant DNAtechniques, a DNA molecule encoding a JIP-1 polypeptide.

“Operably linked” means that the selected DNA molecule is positionedadjacent to one or more sequence elements that direct transcriptionand/or translation of the sequence such that the sequence elements cancontrol transcription and/or translation of the selected DNA (i.e., theselected DNA is operably associated with the sequence elements). Suchoperably associated elements can be used to facilitate the production ofa JIP-1 polypeptide.

The invention also features purified antibodies which specifically binda JIP-1 protein or polypeptide. A “purified antibody” is an antibodywhich is at least 60%, by dry weight, free from the proteins andnaturally-occurring organic molecules with which it is naturallyassociated. The preparation can be at least 75%, at least 90%, and up to99% or more, by dry weight, antibody.

An antibody that “specifically binds” an antigen recognizes and binds tothat antigen, e.g., a JIP-1 polypeptide.

Also within the invention are antisense molecules and ribozymes forinhibiting JIP-1 expression.

The invention also features antagonists and agonists of JIP-1.Antagonists can inhibit one or more of the functions of JIP-1. Suitableantagonists can include large or small molecules, antibodies to JIP-1,and JIP-1 polypeptides that compete with a native form of JIP-1. Suchantagonists include SEQ ID NO:3, a component of an active site of JIP-1,i.e., the JNK-binding domain. Agonists of JIP-1 will enhance orfacilitate one or more of the functions of JIP-1. Agonists andAntagonists include polyproline motifs, which bind to SH3 domains suchas that found in JIP-1.

A “therapeutically effective amount” of a substance is an amount capableof producing a medically desirable result in a treated patient, e.g.,inhibition of the expression or activity of a specific protein.

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe arts of protein chemistry or molecular biology. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of the present invention, suitable methods andmaterials are described infra. All publications, patent applications,patents and other references mentioned herein are incorporated byreference in their entirety. In case of conflict, the presentspecification, including definitions, will control. In addition, thematerials, methods, and examples are illustrative only and are notintended to be limiting.

Other features and advantages of the invention will be apparent from thedetailed description and the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1A is a schematic diagram showing the structure of a murine JIP-1polypeptide.

FIG. 1B is a representation of the amino acid sequence of a murine JIP-1polypeptide (SEQ ID NO:1), presented in single letter code.

FIG. 1C is a representation of the nucleotide sequence of a murine JIP-1cDNA (SEQ ID NO:2).

FIG. 2 is a diagram showing an alignment of the JBDs (JNK-bindingdomains) of JIP-1 (SEQ ID NO:3), c-Jun (SEQ ID NO:5), and ATF2 (SEQ IDNO:6).

FIG. 3 is a diagram showing the amino acid sequences of wild type andmutant JIP-1 peptides (SEQ ID NOs:3 and 7 to 11), as well as a controlpeptide (SEQ ID NO:12).

FIG. 4A is a bar graph showing the effect of recombinant JBD (JIP-1residues 127-281) on reporter gene expression mediated by the GAL4binding domain and GAL4 fusions with the c-myc, Sp1, and VP16 activationdomains.

FIG. 4B is a bar graph showing the effect of recombinant JBD (JIP-1residues 127-281) on reporter gene expression mediated by wild type andmutant forms of GAL4-c-Jun, GAL4-ATF2, and GAL4-Elk-1.

FIG. 4C is a bar graph showing the effect of wild type and mutant JIP-1and JBD on reporter gene expression mediated by wild type and mutantGAL4-ATF2.

FIG. 5 is a bar graph showing the effect of the JBD of JIP-1 on NerveGrowth Factor (NGF) withdrawal-induced apoptosis.

FIG. 6 is a bar graph showing the effect of bicistronic retroviruses onprimary mouse bone marrow cells.

DETAILED DESCRIPTION

The invention is based on the molecular cloning and characterization ofJIP-1, a cytoplasmic protein that specifically binds JNK. JIP-1polypeptides cause cytoplasmic retention of JNK and inhibition ofJNK-regulated gene expression. In addition, JIP-1 polypeptides suppressthe effects of the JNK signaling pathway, including oncogenictransformation and apoptosis. These findings have important implicationsfor the treatment or prevention of pathological conditions and diseases,many of which are characterized by transformation or apoptosis.Conditions associated with apoptosis include neurodegenerativeconditions, such as Parkinson's disease or Alzheimer's disease; andblood clots, which left untreated could result in stroke and associatedmemory loss. Other conditions that can be treated using the compositionsand methods of the invention are autoimmune diseases such as arthritis;other conditions characterized by inflammation; and malignancies, suchas leukemias, e.g., chronic myelogenous leukemia (CML). JIP-1polypeptide compositions can also be used to treat oxidative damage toorgans such as the liver and kidney. Heart disease can also be treatedwith the compositions of the invention. Donor organs for transplantationcan also be treated with JIP-1 compositions.

JIP-1 Proteins and Polypeptides

As shown in FIG. 1, the 660 amino acid JIP-1 protein has an SH3 domainat its carboxy terminal end, at amino acid positions 491-540, and aJNK-binding domain (JBD) at its amino terminal end, at amino acidpositions 127-281 (SEQ ID NO:4). The core of the JBD is amino acids148-174 (SEQ ID NO:3). The JBD of JIP-1 shares conserved residues withthe JNK-binding regions of the transcription factors c-Jun and ATF2, asshown in FIG. 2.

JIP-1 polypeptides can be prepared for a wide range of uses including,but not limited to, generation of antibodies, preparation of reagentsfor diagnostic assays, identification of other molecules involved intransformation or apoptosis, preparation of reagents for use inscreening assays for modulators of apoptosis or transformation, andpreparation of therapeutic agents for treatment of disorders related toapoptosis or transformation.

The invention encompasses, but is not limited to, JIP-1 polypeptidesthat are functionally related to JIP-1 encoded by the nucleotidesequence of FIG. 1C (SEQ ID NO:2). Functionally related polypeptidesinclude any polypeptide sharing a functional characteristic withwildtype JIP-1 protein, e.g., the ability to bind JNK polypeptides or toaffect proliferation or apoptosis. Such functionally related JIP-1polypeptides include, but are not limited to, polypeptides havingadditions or substitutions of amino acid residues within the amino acidsequence encoded by the JIP-1 sequences described herein which result ina silent change, thus producing a functionally equivalent gene product.Amino acid substitutions may be made on the basis of similarity inpolarity, charge, solubility, hydrophobicity, hydrophilicity, and/or theamphipathic nature of the residues involved.

For example, nonpolar (hydrophobic) amino acids include alanine,leucine, isoleucine, valine, proline, phenylalanine, tryptophan, andmethionine; polar neutral amino acids include glycine, serine,threonine, cysteine, tyrosine, asparagine, and glutamine; positivelycharged (basic) amino acids include arginine, lysine, and histidine; andnegatively charged (acidic) amino acids include aspartic acid andglutamic acid.

While random mutations can be made to JIP-1 DNA (using randommutagenesis techniques well known to those skilled in the art) and theresulting mutant JIP-1 proteins can be tested for activity,site-directed mutations of the JIP-1 coding sequence can be engineered(using site-directed mutagenesis techniques well known to those skilledin the art) to generate mutant JIP-1 proteins with increased function,e.g., greater JNK-1 binding and inhibition of transformation.

To design functionally related and functionally variant JIP-1polypeptides, it is useful to distinguish between conserved positionsand variable positions. To preserve JIP-1 function, it is preferablethat conserved residues are not altered. Moreover, alteration ofnon-conserved residues are preferably conservative alterations, e.g., abasic amino acid is replaced by a different basic amino acid. To producealtered function variants, it is preferable to make non-conservativechanges at variable and/or conserved positions. Deletions at conservedand variable positions can also be used to create altered functionvariants. Conserved amino acids in JIP-1 include, but are not limitedto, Lys-155, Thr-159, Leu-160, Asn-161, and Leu-162.

Preferred JIP-1 polypeptides are those that bind JNK and inhibittransformation or apoptosis. These JIP-1 polypeptides have 20%, 40%,50%, 75%, 80%, 90%, or even greater than 100% of the activity of thefull-length JIP-1 described herein. Such comparisons are generally basedon equal concentrations of the molecules being compared. The comparisoncan also be based on the amount of protein or polypeptide required toreach 50% of the maximal stimulation obtainable.

Polypeptides corresponding to one or more domains of JIP-1, e.g., theJNK Binding Domain (JBD), are also within the scope of the invention.Preferred polypeptides are those which are soluble under normalphysiological conditions. Also within the invention are fusion proteinsin which a portion (e.g., one or more domains) of JIP-1 is fused to anunrelated protein or polypeptide (i.e., a fusion partner) to create afusion protein. The fusion partner can be a moiety selected tofacilitate purification, detection, or solubilization, or to providesome other function. Fusion proteins are generally produced byexpressing a hybrid gene in which a nucleotide sequence encoding all ora portion of JIP-1 is joined in-frame to a nucleotide sequence encodingthe fusion partner. Fusion partners include, but are not limited to, theconstant region of an immunoglobulin (IgFc). A fusion protein in which aJIP-1 polypeptide is fused to IgFc can be more stable and have a longerhalf-life in the body than the JIP-1 polypeptide on its own.

Also within the scope of the invention are various soluble forms ofJIP-1, including JIP-1 expressed on its own or fused to a solubilizationpartner, e.g., an immunoglobulin.

In general, JIP-1 polypeptides can be produced by transformation(transfection, transduction, or infection) of a host cell with all orpart of a JIP-1-encoding DNA fragment (e.g., the cDNA described herein)in a suitable expression vehicle. Suitable expression vehicles include:plasmids, viral particles, and phage. For insect cells, baculovirusexpression vectors are suitable. The entire expression vehicle, or apart thereof, can be integrated into the host cell genome. In somecircumstances, it is desirable to employ an inducible expression vector,e.g., the LACSWITCH™ Inducible Expression System (Stratagene; LaJolla,Calif.).

Any of a wide variety of expression systems can be used to provide therecombinant proteins. The precise host cell used is not critical to theinvention. The JIP-1 protein can be produced in a prokaryotic host(e.g., E. coli or B. subtilis) or in a eukaryotic host, e.g., yeast,such as Saccharomyces or Pichia; mammalian cells, such as COS, NIH 3T3,CHO, BHK, 293, or HeLa cells; or insect cells.

JIP-1 polypeptides can also be produced by plant cells. Viral expressionvectors (e.g., cauliflower mosaic virus and tobacco mosaic virus) andplasmid expression vectors (e.g., Ti plasmid) are suitable for use inplant cells. Plant cells are available from a wide range of sources,e.g., the American Type Culture Collection, Rockland, Md. See also,e.g., Ausubel et al., Current Protocols in Molecular Biology, John Wiley& Sons, New York, 1995. The methods of transformation or transfectionand the choice of expression vehicle will depend on the host systemselected. Transformation and transfection methods are described in,e.g., Ausubel et al., supra; expression vehicles may be chosen fromthose described in, e.g., Cloning Vectors: A Laboratory Manual, P. H.Pouwels et al., 1985, Supp. 1987.

The host cells harboring the expression vehicle can be cultured inconventional nutrient media adapted as needed for activation of a chosengene, repression of a chosen gene, selection of transformants, oramplification of a chosen gene.

A suitable expression system is the mouse 3T3 fibroblast host celltransfected with a pMAMneo expression vector (Clontech, Palo Alto,Calif.). pMAMneo provides an RSV-LTR enhancer linked to adexamethasone-inducible MMTV-LTR promotor, an SV40 origin of replicationwhich allows replication in mammalian systems, a selectable neomycingene, and SV40 splicing and polyadenylation sites. DNA encoding a JIP-1in protein would be inserted into the pMAMneo vector in an orientationdesigned to allow expression. The recombinant JIP-1 protein would beisolated as described below. Other preferable host cells that can beused in conjunction with the pMAMneo expression vehicle include COScells and CHO cells (ATCC Accession Nos. CRL 1650 and CCL 61,respectively).

JIP-1 polypeptides can be expressed as fusion proteins. For example, theexpression vector pUR278 can be used to create lacZ fusion proteins. SeeRuther et al., EMBO J. 2:1791 (1983). The pGEX vectors can be used toexpress foreign polypeptides as fusion proteins with glutathioneS-transferase (GST). In general, such fusion proteins are soluble andcan be easily purified from cell lysates by adsorption toglutathione-agarose beads followed by elution in the presence of freeglutathione. The pGEX vectors are designed to include thrombin or factorXa protease cleavage sites so that the cloned target gene product can bereleased from the GST moiety.

In an insect cell expression system, Autographa californica nuclearpolyhidrosis virus (AcNPV), which grows in Spodoptera frugiperda cells,is used as a vector to express foreign genes. A JIP-1 coding sequencecan be cloned individually into non-essential regions (for example thepolyhedrin gene) of the virus and placed under control of a AcNPVpromoter, e.g., the polyhedrin promoter. Successful insertion of a geneencoding a JIP-1 polypeptide or protein will result in inactivation ofthe polyhedrin gene and production of non-occluded recombinant virus(i.e., virus lacking the proteinaceous coat encoded by the polyhedringene). Spodoptera frugiperda cells are then infected with these viruses,and the inserted gene is expressed. See, e.g., Smith et al., J. Virol.46:584 (1983); Smith, U.S. Pat. No. 4,215,051.

In mammalian host cells, a number of viral-based expression systems canbe utilized. In cases where an adenovirus is used as an expressionvector, the JIP-1 nucleic acid sequence can be ligated to an adenovirustranscription/translation control complex, e.g., the late promoter andtripartite leader sequence, to form a chimeric gene. This chimeric genecan then be inserted into the adenovirus genome by in vitro or in vivorecombination. Insertion into a non-essential region of the viral genome(e.g., the E1 or E3 gene) will result in a recombinant virus that isviable and capable of expressing a JIP-1 gene product in infected hosts.See, e.g., Logan, Proc. Natl. Acad. Sci. USA, 81:3655 (1984).

Specific initiation signals may be required for efficient translation ofinserted nucleic acid sequences. These signals include the ATGinitiation codon and adjacent sequences. In cases where an entire nativeJIP-1 gene or cDNA, including its own initiation codon and adjacentsequences, is inserted into the appropriate expression vector, noadditional translational control signals may be needed. In other cases,exogenous translational control signals, including, perhaps, the ATGinitiation codon, must be provided. Furthermore, the initiation codonmust be in phase with the reading frame of the desired coding sequenceto ensure translation of the entire insert. Exogenous translationalcontrol signals and initiation codons can be of a variety of origins,both natural and synthetic. The efficiency of expression may be enhancedby the inclusion of appropriate transcription enhancer elements. Bittneret al., Methods in Enzymol., 153:516 (1987).

In addition, a host cell may be chosen which modulates the expression ofthe inserted sequences, or modifies and processes the gene product in adesired fashion. Such modifications (e.g., glycosylation) and processing(e.g., cleavage) of protein products may be important for the functionof the protein. Different host cells have characteristic and specificmechanisms for post-translational processing and modification ofproteins and gene products. Appropriate cell lines or host systems canbe chosen to ensure the correct modification and processing of theforeign protein expressed. To this end, eukaryotic host cells thatpossess the cellular machinery for proper processing of the primarytranscript, glycosylation, and phosphorylation of the gene product canbe used. Such mammalian host cells include, but are not limited to, CHO,VERO, BHK, HeLa, COS, MDCK, 293, 3T3, and WI38 cells.

Alternatively, a JIP-1 protein can be produced by a stably-transfectedmammalian cell line. A number of vectors suitable for stabletransfection of mammalian cells are available to the public. See, e.g.,Pouwels et al., supra. Methods for constructing such cell lines are alsopublicly available. See, e.g., Ausubel et al., supra. JIP-1 cDNA can becloned into an expression vector that includes the dihydrofolatereductase (DHFR) gene. Methotrexate (0.01-300 μM) is present in theculture medium to select for cells which have integrated the plasmidand, therefore, the JIP-1 cDNA. See Ausubel et al., supra. This dominantselection can be accomplished in most cell types.

Recombinant protein expression can be increased by DHFR-mediatedamplification of the transfected gene. Methods for selecting cell linesbearing gene amplifications are described in Ausubel et al., supra. Suchmethods generally involve extended culture in medium containinggradually increasing levels of methotrexate. DHFR-containing expressionvectors commonly used for this purpose include pCVSEII-DHFR andpAdD26SV(A). See Ausubel et al., supra. Any of the host cells describedabove or, preferably, a DHFR-deficient CHO cell line (e.g., CHO DHFR⁻cells, ATCC Accession No. CRL 9096) are among the host cells preferredfor DHFR selection of a stably-transfected cell line or DHFR-mediatedgene amplification.

A number of other selection systems can be used, including but notlimited to the herpes simplex virus thymidine kinase,hypoxanthine-guanine phosphoribosyltransferase, and adeninephosphoribosyltransferase genes can be employed in tk, hgprt, or aprtcells, respectively. In addition, gpt, which confers resistance tomycophenolic acid (Mulligan et al., Proc. Natl. Acad. Sci. USA, 78:2072(1981); neo, which confers resistance to the aminoglycoside G-418(Colberre-Garapin et al., J. Mol. Biol., 150:1 (1981)); and hygro, whichconfers resistance to hygromycin (Santerre et al., Gene, 30:147 (1981)),can be used.

Alternatively, any fusion protein can be readily purified by utilizingan antibody specific for the fusion protein being expressed. Forexample, a system described in Janknecht et al., Proc. Natl. Acad. Sci.USA, 88:8972 (1981), allows for the ready purification of non-denaturedfusion proteins expressed in human cell lines. In this system, the geneof interest is subcloned into a vaccinia recombination plasmid such thatthe gene's open reading frame is translationally fused to anamino-terminal tag consisting of six histidine residues. Extracts fromcells infected with recombinant vaccinia virus are loaded onto Ni²⁺nitriloacetic acid-agarose columns, and histidine-tagged proteins areselectively eluted with imidazole-containing buffers.

Alternatively, JIP-1 or a portion thereof can be fused to animmunoglobulin Fc domain. Such a fusion protein can be readily purifiedusing a protein A column. Moreover, such fusion proteins permit theproduction of a dimeric form of a JIP-1 polypeptide having increasedstability in vivo.

After the recombinant JIP-1 protein is expressed, it is isolated.Secreted forms can be isolated from the culture media, whilenon-secreted forms must be isolated from the host cells. Proteins can beisolated by affinity chromatography. An anti-JIP-1 antibody (e.g.,produced as described herein) is attached to a column and used toisolate the JIP-1 protein. Lysis and fractionation of JIP-1protein-harboring cells prior to affinity chromatography can beperformed by standard methods. See, e.g., Ausubel et al., supra.Alternatively, a JIP-1 fusion protein, for example, a JIP-1-maltosebinding protein, a JIP-1-β-galactosidase, or a JIP-1-trpE fusionprotein, can be constructed and used for JIP-1 protein isolation. See,e.g., Ausubel et al., supra; New England Biolabs, Beverly, Mass.

Once isolated, the recombinant protein can, if desired, be furtherpurified, e.g., by high performance liquid chromatography using standardtechniques. See, e.g., Fisher, Laboratory Techniques In Biochemistry AndMolecular Biology, Work et al., eds., Elsevier (1980).

Polypeptides of the invention, particularly short JIP-1 fragments, canalso be produced by chemical synthesis, e.g., by the methods describedin Solid Phase Peptide Synthesis, 2nd ed., The Pierce Chemical Co.,Rockford, Ill., 1984.

The invention also features proteins which interact with JIP-1 and whichare involved in the function of JIP-1. Also included in the inventionare the genes encoding these interacting proteins. Interacting proteinscan be identified using methods known to those skilled in the art. Onemethod suitable method is the “two-hybrid system” which detects proteininteractions in vivo. See, e.g., Chien et al., Proc. Natl. Acad. Sci.USA, 88:9578 (1991). A kit for practicing this method is available fromClontech (Palo Alto, Calif.).

JIP-1 Nucleic Acids

The JIP-1 cDNA sequences described herein, and related family members ofthe JIP-1 gene present in mouse, human, or other species can beidentified and readily isolated without undue experimentation by wellknown molecular biological techniques given the specific sequencesdescribed herein. Further, genes may exist at other loci that encodeproteins having extensive homology to JIP-1 polypeptides or one or moredomains of JIP-1 polypeptides. These genes can be identified by knowntechniques using the sequences disclosed herein. For example,hybridization of JIP-1 probes to homologous nucleic acids is performedunder stringent conditions. Alternatively, a labeled fragment can beused to screen a genomic library derived from the organism of interest,again, using appropriately stringent conditions. Such stringentconditions are well known, and will vary predictably depending on thespecific organisms from which the library and the labeled sequences arederived.

Nucleic acid duplex or hybrid stability is expressed as the meltingtemperature, or T_(m), which is the temperature at which a probedissociates from a target DNA. This melting temperature is used todefine the required stringency conditions. If sequences are to beidentified that are related and substantially identical to the probe,rather than identical, then it is useful to first establish the lowesttemperature at which only homologous hybridization occurs with aparticular concentration of SSC or SSPE. It is then assumed that 1%mismatching results in a 1° C. decrease in the T_(m), and thetemperature of the final wash is reduced accordingly (for example, ifsequences with ≧95% identity with the probe are sought, the final washtemperature is decreased by 5° C.). Note that this assumption is veryapproximate, and the actual change in T_(m) can be between 0.5° and1.50° C. per 1% mismatch.

As used herein, stringent conditions include hybridization at 68° C. in5×SSC/5×Denhardt's solution/1.0% SDS, or in 0.5 M NaHPO₄ (pH 7.2)/1 mMEDTA/7% SDS, or in 50% formamide/0.25 M NaHPO₄ (pH 7.2)/0.25 M NaCl/1 mMEDTA/7% SDS; and washing in 0.2×SSC/0.1% SDS at room temperature or at42° C., or in 0.1×SSC/0.1% SDS at 68° C., or in 40 mM NaHPO₄ (pH 7.2)/1mM EDTA/5% SDS at 50° C., or in 40 mM NaHPO₄ (pH 7.2) 1 mM EDTA/1% SDSat 50° C. Moderately stringent conditions include washing in 3×SSC at42° C. The parameters of salt concentration and temperature can bevaried to achieve the desired level of identity between the probe andthe target nucleic acid. For guidance regarding such conditions see,e.g., Sambrook et al., Molecular Cloning, A Laboratory Manual, ColdSpring Harbor Press, Cold Spring Harbor, N.Y.; and Ausubel et al.,supra, at Unit 2.10.

Upon detection of JIP-1 transcript in human cell lines by Northern blotanalysis, cDNA libraries can be constructed from RNA isolated from thesecell lines utilizing standard techniques. The human cDNA library canthen be screened with a JIP-1 probe to isolate a human JIP-1 cDNA.

Alternatively, a human genomic DNA library can be screened using JIP-1probes. Hybridizing clones can be sequenced and the intron and exonstructure of the human JIP-1 gene can be elucidated. Once a genomicsequence is obtained, oligonucleotide primers can be designed based onthe sequence for use in Reverse Transcriptase-coupled PCR, e.g., toisolate human JIP-1 cDNA. An example of a suitable probe for screening ahuman genomic library is the coding region (nucleotides 180 to 2159) ofthe mouse JIP-1 cDNA (SEQ ID NO:2).

Further, a previously unknown gene sequence can be isolated byperforming PCR using two degenerate oligonucleotide primer poolsdesigned on the basis of nucleotide sequences within the JIP-1 cDNAdefined herein. Degenerate PCR primers that can be used include:

5′ GARGARTTYGARGAYGARGA 3′ (sense; SEQ ID NO:25);

5′ GGNAARAARCAYAGNTGGCA 3′ (sense; SEQ ID NO:26);

5′ CATRTTWTANGCYTCWTACCA 3′ (antisense; SEQ ID NO:27); and

5′ AAYTGYTTKTARAAYTGYTGRAA 3′ (antisense; SEQ ID NO:28),

where W is A or T, K is G or T, R is A or G, Y is C or T, and N is A, C,G or T. The template for the reaction can be cDNA obtained by reversetranscription of mRNA prepared from human or non-human cell lines ortissue known to express, or suspected of expressing, JIP-1. The PCRproduct can be subcloned and sequenced to insure that the amplifiedsequences represent the sequences of JIP-1 or JIP-1-like gene nucleicacid sequences.

The PCR fragment can then be used to isolate a full length cDNA clone bya variety of methods. For example, the amplified fragment can be labeledand used to screen a cDNA library in bacteriophage. Alternatively, thelabeled fragment can be used to screen a genomic library.

PCR technology also can be used to isolate full length cDNA sequences.For example, RNA can be isolated, following standard procedures, from anappropriate tissue or cell line. A reverse transcription reaction can beperformed on the RNA using an oligonucleotide primer specific for themost 5′ end of the amplified fragment for the priming of first strandsynthesis. The resulting RNA/DNA hybrid can then be “tailed” withguanines using a standard terminal transferase reaction. After digestionwith RNAase H, and second strand synthesis can be primed with a poly-Cprimer. Thus, cDNA sequences upstream of the amplified fragment caneasily be isolated. For a review of useful cloning strategies, see e.g.,Sambrook et al., supra; Ausubel et al., supra.

Mutant cDNAs can also be isolated using PCR techniques. The first cDNAstrand can be synthesized by hybridizing an oligo-dT oligonucleotide tomRNA isolated from tissue known to be or suspected of being expressed inan individual putatively carrying the mutant allele, and by extendingthe new strand with reverse transcriptase. The second strand of the cDNAcan then be synthesized using an oligonucleotide that hybridizesspecifically to the 5′—end of the normal gene. Using these twooligonucleotides as primers, the product is amplified via PCR, clonedinto a suitable vector, and subjected to DNA sequence analysis bymethods well known in the art. By comparing the DNA sequence of themutant gene to that of the normal gene, the mutation(s) responsible forthe loss or alteration of function of the mutant gene product can beascertained.

Alternatively, a genomic or cDNA library can be constructed and screenedusing DNA or RNA, respectively, from a tissue known to express, orsuspected of expressing, the gene of interest in an individual suspectedof carrying or known to carry the mutant allele. The normal gene or anysuitable fragment thereof can then be labeled and used as a probe toidentify the corresponding mutant allele in the library. The clonecontaining the mutant gene can then be purified through methodsroutinely practiced in the art, and subjected to sequence analysis usingstandard techniques as described herein.

Additionally, an expression library can be constructed using DNAisolated from or cDNA synthesized from a tissue known to express orsuspected of expressing the gene of interest in an individual suspectedof carrying or known to carry the mutant allele. In this manner, geneproducts made by this tissue can be expressed and screened usingstandard antibody screening techniques in conjunction with antibodiesraised against the normal gene product, as described herein. Forscreening techniques, see, for example, Harlow et al., eds., Antibodies:A Laboratory Manual, Cold Spring Harbor Press, Cold Spring Harbor, N.Y.(1988).

In cases where the mutation results in an expressed gene product withaltered function (e.g., as a result of a missense mutation), apolyclonal set of antibodies is likely to cross-react with the mutantgene product. Library clones detected via their reaction with suchlabeled antibodies can be purified and subjected to sequence analysis asdescribed herein.

JIP-1 Peptide Mimetics

JIP-1 peptide mimetics can be constructed by structure-based drug designthrough replacement of amino acids by organic moieties. See, e.g.,Hughes, Philos. Trans. R. Soc. Lond., 290:387-394 (1980); Hodgson,Biotechnol., 9:19-21 (1991); Suckling, Sci. Prog., 75:323-359 (1991).The use of peptide mimetics can be enhanced through the use ofcombinatorial chemistry to create drug libraries. The design of peptidemimetics can be aided by identifying amino acid mutations that increaseor decrease JIP-1 binding to JNK. Approaches that can be used includethe yeast two hybrid method (see, e.g., Chien et al., Proc. Natl. Acad.Sci. USA, 88:9578 (1991); kit from Clontech, Palo Alto, Calif.), and thephage display method. The two hybrid method detects protein-proteininteractions in yeast. Fields et al., Nature, 340:245-246 (1989). Thephage display method detects the interaction between an immobilizedprotein and a protein that is expressed on the surface of phages (e.g.lambda and M13). Amberg, et al., Strategies, 6:2-4 (1993); Hogrefe etal., Gene, 128:119-126 (1993). These methods allow positive and negativeselection for protein-protein interactions and the identification of thesequences that determine these interations.

Transgenic Animals

JIP-1 polypeptides can also be expressed in transgenic animals. Animalsof any species, including, but not limited to, mice, rats, rabbits,guinea pigs, pigs, micro-pigs, goats, and non-human primates, e.g.,baboons, monkeys, and chimpanzees, can be used to generateJIP-1-expressing transgenic animals.

Various techniques known in the art can be used to introduce a JIP-1transgene into animals to produce the founder lines of transgenicanimals. Such techniques include, but are not limited to, pronuclearmicroinjection (U.S. Pat. No. 4,873,191); retrovirus mediated genetransfer into germ lines (Van der Putten et al., Proc. Natl. Acad. Sci.USA, 82:6148 (1985)); gene targeting into embryonic stem cells (Thompsonet al., Cell, 56:313 (1989)); and electroporation of embryos (Lo, Mol.Cell. Biol, 3:1803 (1983)).

The present invention provides for transgenic animals that carry theJIP-1 transgene in all their cells, as well as animals that carry thetransgene in some, but not all of their cells, i.e., mosaic animals. Thetransgene can be integrated as a single transgene or in concatamers,e.g., head-to-head tandems or head-to-tail tandems. The transgene canalso be selectively introduced into and activated in a particular celltype. Lasko et al., Proc. Natl. Acad. Sci. USA, 89:6232 (1992). Theregulatory sequences required for such a cell-type specific activationwill depend upon the particular cell type of interest, and will beapparent to those of skill in the art.

When it is desired that the JIP-1 transgene be integrated into thechromosomal site of the endogenous JIP-1 gene, gene targeting ispreferred. Vectors containing some nucleotide sequences homologous to anendogenous JIP-1 gene are designed for the purpose of integrating viahomologous recombination into the endogenous gene and disrupting itsfunction. The transgene also can be selectively introduced into aparticular cell type, thus inactivating the endogenous JIP-1 gene inonly that cell type. See Gu et al., Science, 265:103 (1984). Theregulatory sequences required for such a cell-type specific inactivationwill depend upon the particular cell type of interest, and will beapparent to those of skill in the art.

Once transgenic animals have been generated, the expression of therecombinant JIP-1 gene can be assayed utilizing standard techniques.Initial screening can be accomplished by Southern blot analysis or PCRtechniques to analyze animal tissues to assay whether integration of thetransgene has taken place. The level of mRNA expression of the transgenein the tissues of the transgenic animals can also be assessed usingtechniques which include, but are not limited to, Northern blot analysisof tissue samples obtained from the animal, in situ hybridizationanalysis, and RT-PCR. Samples of tissues expressing JIP-1 can also beevaluated immunocytochemically using antibodies specific for the JIP-1transgene product.

Anti-JIP-1 Antibodies

Since JIP-1 is an inhibitor of JNK, inhibition of JIP-1 increases JNKactivity. Increased JNK expression results in increased apoptosis, e.g.,in neurons. Induction of apoptosis would be desirable in brain tumors,for example. Therefore, antibodies specific for JIP-1 can be used toinhibit JIP-1 expression.

Human JIP-1 proteins and polypeptides (or immunogenic fragments oranalogs) can be used to raise antibodies; such polypeptides can beproduced by recombinant or peptide synthetic techniques. See, e.g.,Solid Phase Peptide Synthesis, supra; Ausubel et al., supra. In general,the peptides can be coupled to a carrier protein, such as KLH, mixedwith an adjuvant, and injected into a host mammal. Antibodies can bepurified by peptide antigen affinity chromatography.

In particular, various host animals can be immunized by injection with aJIP-1 protein or polypeptide. Host animals include rabbits, mice, guineapigs, and rats. Various adjuvants can be used to increase theimmunological response, depending on the host species, including but notlimited to Freund's (complete or incomplete); mineral gels, such asaluminum hydroxide; surface active substances such as lysolecithin;pluronic polyols; polyanions; peptides; oil emulsions; keyhole limpethemocyanin; dinitrophenol; and potentially useful human adjuvants suchas BCG (bacillus Calmette-Guerin) and Corynebacterium parvum. Polyclonalantibodies are heterogeneous populations of antibody molecules derivedfrom the sera of the immunized animals.

The invention includes monoclonal antibodies, polyclonal antibodies,humanized or chimeric antibodies, single chain antibodies, Fabfragments, F(ab′)₂ fragments, and molecules produced using a Fabexpression library.

Monoclonal antibodies, which are homogeneous populations of antibodiesto a particular antigen, can be prepared using the JIP-1 polypeptidesdescribed above and standard hybridoma technology. See, e.g., Kohler etal., Nature, 256:495 (1975); Kohler et al., Eur. J. Immunol., 6:511(1976); Kohler et al., Eur. J. Immunol., 6:292 (1976); Hammerling etal., In Monoclonal Antibodies and T Cell Hybridomas, Elsevier, N.Y.(1981); Ausubel et al., supra.

In particular, monoclonal antibodies can be obtained by any techniquethat provides for the production of antibody molecules by continuouscell lines in culture. Such methods include those described in Kohler etal., Nature 256:495 (1975), and U.S. Pat. No. 4,376,110. Other methodsof producing monoclonal antibodies include the human B-cell hybridomatechnique (Kozbor et al., Immunology Today 4:72 (1983); Cole et al.,Proc. Natl. Acad. Sci. USA 80:2026 (1983), and the EBV-hybridomatechnique (Cole et al., Monoclonal Antibodies and Cancer Therapy, AlanR. Liss, Inc., pp. 77-96 (1983)). Such antibodies can be of anyimmunoglobulin class, e.g., IgG, IgM, IgE, IgA, IgD, and any subclassthereof. Hybridomas producing the monoclonal antibodies of the inventionmay be cultivated in vitro or in vivo. The ability to produce hightiters of monoclonal antibodies in vivo makes this the presentlypreferred method of production.

Polyclonal or monoclonal antibodies are tested for specific JIP-1recognition by Western blot or immunoprecipitation analysis by standardmethods. See, e.g., Ausubel et al., supra. Antibodies that specificallyrecognize and bind to JIP-1 are useful in the invention. Theseantibodies can be used in immunoassays to monitor the level of JIP-1produced by a mammal, e.g., to determine the amount or subcellularlocation of JIP-1).

Preferably, antibodies of the invention are produced using JIP-1polypeptides that correspond to regions of the JIP-1 protein that lieoutside highly conserved regions and appear likely to be antigenic, bycriteria such as high frequency of charged residues. Such fragments canbe generated by standard PCR techniques and then cloned into the pGEXexpression vector. Ausubel et al., supra. Fusion proteins are expressedin E. coli and purified using a glutathione agarose affinity matrix asdescribed in Ausubel, et al., supra.

In some cases it may be desirable to minimize the potential problems oflow affinity or specificity of antisera. In such circumstances, two orthree fusion proteins can be generated for each protein, and each fusionprotein can be injected into at least two rabbits. Antisera can beraised by injections in a series, preferably including at least threebooster injections.

Antisera is also checked for its ability to immunoprecipitaterecombinant JIP-1 proteins or control proteins, such as glucocorticoidreceptor, CAT, or luciferase.

The antibodies can be used to detect JIP-1 in a biological sample aspart of a diagnostic assay. Antibodies also can be used in a screeningassay to measure the effect of a candidate compound on expression orlocalization of JIP-1. Additionally, such antibodies can be used inconjunction with the gene therapy techniques, e.g., to evaluate thenormal and/or engineered JIP-1-expressing cells prior to theirintroduction into the patient. Such antibodies additionally can be usedin a method for inhibiting abnormal JIP-1 activity. Such abnormalactivity includes altered apoptosis and proliferation, resulting inneurodegenerative diseases, autoimmune disease, cancers such asleukemias. Other abnormal JIP-1 activity includes damage caused byischemia/reperfusion, especially in heart disease, kidney damage, andstroke.

For theraputic uses, murine or other monoclonal antibodies should bealtered to make them less immunogenic when administered to humanpatients. For example, techniques have been developed for the productionof “chimeric antibodies.” See Morrison et al., Proc. Natl. Acad. Sci.,81:6851 (1984); Neuberger et al., Nature, 312:604 (1984); Takeda et al.,Nature, 314:452 (1984). These techniques involve splicing the genes froma mouse antibody molecule of appropriate antigen specificity togetherwith genes from a human antibody of appropriate biological activity.Such chimeric antibodies have, e.g., a variable region derived from amurine antibody and a constant region derived from a human antibody.

Alternatively, single chain antibodies specific for JIP-1 polypeptidescan be produced using known techniques. See, e.g., U.S. Pat. Nos.4,946,778 and 4,704,692. Single chain antibodies are formed by linkingthe heavy and light chain fragments of the Fv region via an amino acidbridge, resulting in a single chain polypeptide.

Antibody fragments that recognize and bind to specific epitopes of JIP-1can also be generated by known techniques. Such fragments include, butare not limited to, F(ab′)₂ fragments, produced by pepsin digestion ofantibody molecules, and Fab fragments, generated by reduction of thedisulfide bonds of F(ab′)₂ fragments. Alternatively, Fab expressionlibraries can be constructed to allow rapid and easy identification ofmonoclonal Fab fragments with a desired specificity. See, e.g., Huse etal., Science 246:1275 (1989).

Antibodies to JIP-1 can be used to generate anti-idiotypic antibodiesthat resemble a portion of JIP-1, using techniques well known to thoseskilled in the art. See, e.g., Greenspan et al., FASEB J. 7:437 (1993);Nisonoff, J. Immunol. 147:2429 (1991). For example, antibodies that bindto JIP-1 and competitively inhibit binding of other ligands can be usedto generate anti-idiotypic antibodies resembling a ligand binding domainof JIP-1. These anti-idiotypic antibodies can bind and neutralize JIP-1ligands. JIP-1 ligands include proline-rich regions of proteins, sinceJIP-1 has an SH3 domain that binds to these regions. There are tenisoforms of JNK. Gupta et al., EMBO J. 15:2760-2770 (1996). Each ofthese JNK isoforms is a JIP-1 ligand. Other kinases, which may berelated to JNK, may also interact with JIP-1. Neutralizinganti-idiotypic antibodies or Fab fragments of anti-idiotypic antibodiescan be used in therapeutic regimens.

Antisense Nucleic Acids

In alternate embodiments, therapies can be designed to reduce the levelof endogenous JIP-1 gene expression, e.g., using antisense or ribozymeapproaches to inhibit or prevent translation of JIP-1 mRNA transcripts;triple helix approaches to inhibit transcription of the JIP-1 gene; ortargeted homologous recombination to inactivate or “knock out” the JIP-1gene or its endogenous promoter. Delivery techniques can be designed toallow theraputic compositions to cross the blood-brain barrier (see,e.g., PCT WO89/10134).

Antisense approaches involve the design of oligonucleotides (either DNAor RNA) that are complementary to JIP-1 mRNA and inhibit expression ofJIP-1 protein. Absolute complementarity of the antisense oligonucleotideto JIP-1, although preferred, is not required. A sequence“complementary” to a portion of an RNA, as referred to herein, means asequence having sufficient complementarity to be able to hybridize withthe RNA, forming a stable duplex; in the case of double-strandedantisense nucleic acids, a single strand of the duplex DNA may betested, or triplex formation may be assayed. The ability to hybridizewill depend on both the degree of complementarily and the length of theantisense nucleic acid. Generally, the longer the hybridizing nucleicacid, the more base mismatches with an RNA it may contain and still forma stable duplex (or triplex, as the case may be). One skilled in the artcan ascertain a tolerable degree of mismatch by use of standardprocedures to determine the melting point of the hybridized complex.

Oligonucleotides that are complementary to the 5′ end of the message,e.g., the 5′ untranslated sequence up to and including the AUGinitiation condon, should work most efficiently at inhibitingtranslation. However, sequences complementary to the 3′ untranslatedsequences of mRNAs have been shown to be effective at inhibitingtranslation of mRNAs as well (Wagner, Nature, 372:333, 1994). Thus,oligonucleotides complementary to either the 5′- or 3′-non-translated,non-coding regions of the JIP-1 gene can be used in an antisenseapproach to inhibit translation of endogenous JIP-1 mRNA.Oligonucleotides complementary to the 5′ region overlapping theinitiation codon can be used for this purpose. Antisenseoligonucleotides that are complementary to the coding region can also beused. In designing suitable oligonucleotides, target regions aregenerally those lacking predicted secondary structure.

Antisense oligonucleotides complementary to mRNA coding regions can alsobe used in accordance with the invention. Whether designed to hybridizeto the 5′, 3′, or coding region of JIP-1 mRNA, antisense nucleic acidsshould be at least six nucleotides in length, and are preferablyoligonucleotides ranging from 6 to about 50 nucleotides in length. Inspecific aspects the oligonucleotide is at least 10 nucleotides, atleast 17 nucleotides, at least 25 nucleotides or at least 50 nucleotidesin length.

It is preferred that in vitro studies are first performed to quantitatethe ability of the antisense oligonucleotide to inhibit gene expression.These studies preferably utilize controls that distinguish betweenantisense inhibition and nonspecific biological effects ofoligonucleotides. It is also preferred that these studies compare levelsof the target RNA or protein with that of an internal control RNA orprotein. Additionally, it is envisioned that results obtained using theantisense oligonucleotide are compared with those obtained using acontrol oligonucleotide. It is preferred that the controloligonucleotide is of approximately the same length as the testoligonucleotide and that the nucleotide sequence of the oligonucleotidediffers from the antisense sequence no more than is necessary to preventspecific hybridization to the target sequence.

The oligonucleotides can be DNA or RNA or chimeric mixtures orderivatives or modified versions thereof, single-stranded ordouble-stranded. Stability of the oligonucleotide can be improved bymodification of the phosphodiester moieties by replacement withphosphorothioate, —CH2—, or —NH— groups. The oligonucleotides can alsobe stabilized by blocking the 5′ and 3′ termini with phosphorothioate,—CH2— or —NH— groups. Each of these modifications will lead to increasedstability (e.g. by increasing modifications will lead to increasedstability (e.g. by increasing resistance to nucleases) and thereforeincreased hybridization. The oligonucleotide can be modified at the basemoiety, sugar moiety, or phosphate backbone. The oligonucleotide mayinclude other appended groups such as peptides (e.g., for targeting hostcell receptors in vivo), or agents facilitating transport across thecell membrane (as described, e.g., in Letsinger et al., Proc. Natl.Acad. Sci. USA, 86:6553 (1989); Lemaitre et al., Proc. Natl. Acad. Sci.USA, 84:648 (1987)); PCT Publication No. WO 88/09810) or the blood-brainbarrier (see, e.g., PCT Publication No. WO 89/10134), orhybridization-triggered cleavage agents (see, e.g., Krol et al.,BioTechniques 6:958 (1988)), or intercalating agents (see e.g., Zon,Pharm. Res. 5:539 (1988)). To this end, the oligonucleotide can beconjugated to another molecule, e.g., a peptide, hybridization triggeredcross-linking agent, transport agent, or hybridization-triggeredcleavage agent.

The antisense oligonucleotide can include at least one modified basemoiety which is selected from the group including, but not limited to,5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil,hypoxanthine, xantine, 4-acetylcytosine,5-(carboxyhydroxylmethyl)uracil,5-carboxymethylaminomethyl-2-thiouridine,5-carboxymethylaminomethyluracil, dihydrouracil,beta-D-galactosylqueosine, inosine, N6-isopentenyladenine,1-methylcytosine, N6-adenine, 7-methylguanine,5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil,beta-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 5-methoxyuracil,2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v),wybutoxosine, pseudouracil, queosine, 2-thiocytosine,5-methyl-2-theouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil,uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v),5-methyl-2-thiouracil, 2-(3-amino-3-N-2-carboxypropl)uracil, (acp3)w,and 2,6-diaminopurine.

The antisense oligonucleotide can also include at least one modifiedsugar moiety selected from the group including, but not limited to,arabinose, 2-fluoroarabinose, xylulose, and hexose.

In yet another embodiment, the antisense oligonucleotide includes atleast one modified phosphate backbone selected from the group consistingof a phosphorothioate, a phosphorodithioate, a phosphoramidothioate, aphosphoramidate, a phosphordiamidate, a methylphosphonate, an alkylphosphotriester, and a formacetal, or an analog of any of thesebackbones.

In yet another embodiment, the antisense oligonucleotide is aná-anomeric oligonucleotide. An á-anomeric oligonucleotide forms specificdouble-stranded hybrids with complementary RNA in which, contrary to theusual â-units, the strands run parallel to each other (Gautier et al.,Nucl. Acids. Res., 15:6625 (1987)). The oligonucleotide is a2′-0-methylribonucleotide (Inoue et al., Nucl. Acids Res., 15:6131(1987)), or a chimeric RNA-DNA analog (Inoue et al., FEBS Lett., 215:327(1987)).

Antisense oligonucleotides of the invention can be synthesized by.standard methods known in the art, e.g., by use of an automated DNAsynthesizer (commercially available from Biosearch, Applied Biosystems,etc.). As examples, phosphorothioate oligonucleotides can be synthesizedby the method of Stein et al. (Nucl. Acids Res., 16:3209 (1988)), andmethylphosphonate oligonucleotides can be prepared by use of controlledpore glass polymer supports (Sarin et al., Proc. Natl. Acad. Sci. USA,85:7448 (1988)).

The antisense molecules should be delivered to cells that express JIP-1in vivo. A number of methods have been developed for deliveringantisense DNA or RNA to cells. For example, antisense molecules can beinjected directly into specific tissue sites. Modified antisensemolecules, designed to target the desired cells (e.g., antisense linkedto peptides or antibodies that specifically bind receptors or antigensexpressed on the target cell surface) can be administered systemically.

One approach to achieving intracellular concentrations of the antisensemolecule sufficient to suppress translation of endogenous mRNAs is touse a recombinant DNA construct in which the antisense oligonucleotideis placed under the control of a strong pol III or pol II promoter. Theuse of such a construct to transfect target cells in a patient willresult in the transcription of sufficient amounts of single strandedRNAs that will form complementary base pairs with the endogenous JIP-1transcripts, and thereby prevent translation of the JIP-1 mRNA. Forexample, a vector can be introduced in vivo such that it is taken up bya cell and directs the transcription of an antisense RNA. Such a vectorcan remain episomal or become chromosomally integrated, as long as itcan be transcribed to produce the desired antisense RNA.

Such vectors can be constructed by recombinant DNA technology methodsstandard in the art. Vectors can be plasmid, viral, or others known inthe art, used for replication and expression in mammalian cells.Expression of the sequence encoding the antisense RNA can be by anypromoter known in the art to act in mammalian, preferably human cells.Such promoters can be inducible or constitutive. Such promoters include,but are not limited to: the SV40 early promoter region (Bernoist et al.,Nature, 290:304 (1981)); the promoter contained in the 3′ long terminalrepeat of Rous sarcoma virus (Yamamoto et al., Cell, 22:787-797 (1988));the herpes thymidine kinase promoter (Wagner et al., Proc. Natl. Acad.Sci USA, 78:1441, 1981); or the regulatory sequences of themetallothionein gene (Brinster et al., Nature, 296:39 (1988)).

Any type of plasmid, cosmid, YAC, or viral vector can be used to preparethe recominant DNA construct which can be introduced directly intospecific tissue sites. Alternatively, viral vectors can be used thatselectively infect the desired tissue (e.g., for brain, herpesvirusvectors may be used), in which case administration can be accomplishedby another route (e.g., systemically).

Ribozymes

Ribozyme molecules designed to catalytically cleave JIP-1 mRNAtranscripts also can be used to prevent translation of JIP-1 mRNA andexpression of JIP-1 protein (see, e.g., PCT Publication WO 90/11364;Saraver et al., Science, 247:122 (1990)). While various ribozymes thatcleave mRNA at site-specific recognition sequences can be used todestroy JIP-1 mRNAs, the use of hammerhead ribozymes is preferred.Hammerhead ribozymes cleave mRNAs at locations dictated by flankingregions that form complementary base pairs with the target mRNA. Thesole requirement is that the target mRNA contain the following sequenceof two bases: 5′-UG-3′. The construction and production of hammerheadribozymes is well known in the art (Hasseloff et al., Nature, 334;585,1988).

The ribozymes of the present invention also include RNAendoribonucleases (hereinafter “Cech-type ribozymes”), such as the onethat occurs naturally in Tetrahymena Thermophila (known as the IVS orL-19 IVS RNA), which have been extensively characterized by Cech and hiscollaborators. See, e.g., Zaug et al., Science, 224:574 (1984); Zaug etal., Science, 231:470 (1986); Zaug et al., Nature, 324:429 (1986); PCTApplication No. WO 88/04300; and Been et al., Cell, 47:207 (1986). TheCech-type ribozymes have an eight base-pair sequence that hybridizes toa target RNA sequence, whereafter cleavage of the target RNA takesplace. The invention encompasses those Cech-type ribozymes that targeteight base-pair active site sequences present in JIP-1. In general,target sites for ribozymes are regions that are predicted to lackappreciable secondary structure. Target sites for ribozymes include:

5′ GGUAUCGAUAAGCUUGAUAUCGCUGUCCGGAGC 3′ (SEQ ID NO:29) and

5′ AGAGGCACUGUCCCAUCCUGGGCCUGUUUCAUG 3′ (SEQ ID NO:30).

As in the antisense approach, the ribozymes can be composed of modifiedoligonucleotides (e.g., for improved stability, targeting, etc.), andshould be delivered to cells which express JIP-1 in vivo. A preferredmethod of delivery involves using a DNA construct “encoding” theribozyme under the control of a strong constitutive pol III or pol IIpromoter, so that transfected cells will produce sufficient quantitiesof the ribozyme to destroy endogenous JIP-1 messages and inhibittranslation. Because ribozymes, unlike antisense molecules, arecatalytic, a lower intracellular concentration is required forefficiency. Destruction of JIP-1 mRNA would be advantageous inincreasing apoptosis in tumors; in altering the cell damage occurringduring ischemic reperfusion in cardiovascular disease, kidney disease,and stroke; and altering cell damage occurring in the liver in responseto metabolic oxidative stress.

Other Methods for Reducing JIP-1 Expression

Endogenous JIP-1 gene expression can also be reduced by inactivating or“knocking out” the JIP-1 gene or its promoter using targeted homologousrecomination (see, e.g., U.S. Pat. No. 5,464,764). For example, amutant, non-functional JIP-1 (or a completely unrelated DNA sequence)flanked by DNA homologous to the endogenous JIP-1 gene (either thecoding regions or regulatory regions of the JIP-1 gene) can be used,with or without a selectable marker and/or a negative selectable marker,to transfect cells that express JIP-1 in vivo. Insertion of the DNAconstruct, via targeted homologous recombination, results ininactivation of the JIP-1 gene. Such approaches are particularly suitedfor use in the agricultural field where modifications to ES (embryonicstem) cells can be used to generate animal offspring with an inactiveJIP-1. However, this approach can be adapted for use in humans, providedthe recombinant DNA constructs are directly administered or targeted tothe required site in vivo using appropriate viral vectors, e.g., herpesvirus vectors for delivery to brain tissue.

Alternatively, endogenous JIP-1 gene expression can be reduced bytargeting deoxyribonucleotide sequences complementary to regulatoryregions of the JIP-1 gene (e.g., a JIP-1 promoter and/or enhancer toform triple helical structures that prevent transcription of the JIP-1gene in target cells in the body. See, e.g., Helene, Anticancer DrugDes. 6:569 (1981); Helene et al., Ann N.Y. Acad. Sci. 660:27 (1992);Maher, Bioassays 14:807 (1992).

Identification of Proteins that Interact with JIP-1

The invention also features polypeptides that interact with JIP-1. Anymethod suitable for detecting protein-protein interactions can beemployed for identifying intracellular or extracellular proteins thatinteract with JIP-1. Among the traditional methods that can be employedare co-immunoprecipitation, crosslinking, and co-purification throughgradients or chromatographic columns of cell lysates, or proteinsobtained from cell lysates. Purified proteins are used to identifyproteins in the lysate that interact with JIP-1. For these assays, theJIP-1 polypeptide can be full length JIP-1, a soluble domain of JIP-1,or some other suitable JIP-1 polypeptide.

To characterize JIP-1 interacting proteins, portions of their amino acidsequences can be ascertained using techniques well known to those ofskill in the art, such as the Edman degradation technique. The aminoacid sequences obtained can be used to design degenerate oligonucleotideprobes that can be used to screen for gene sequences encoding theinteracting protein. Screening may be accomplished, for example, bystandard hybridization or PCR techniques. Techniques for the generationof degenerate oligonucleotide mixtures and screening are well-known. SeeAusubel, supra; and Innis et al., eds., PCR Protocols: A Guide toMethods and Applications, Academic Press, Inc., New York (1990).

Additionally, methods can be used that result directly in theidentification of genes that encode proteins that interact with JIP-1.These methods include, for example, screening expression libraries in amanner similar to the well known technique of antibody probing of λgt11libraries using labeled JIP-1 polypeptides or a JIP-1 fusion protein,e.g., an JIP-1 polypeptide or domain fused to a marker such as anenzyme, a fluorescent dye, a luminescent protein, or to an IgFc domain.

Protein interactions can also be identified in vivo using the two-hybridsystem. Chien et al., Proc. Natl. Acad. Sci. USA, 88:9578 (1991). A kitfor practicing this method is available from Clontech (Palo Alto,Calif.). In this system, two plasmids, each encoding a hybrid protein,are constructed. One plasmid, the “bait” plasmid, includes a nucleotidesequence encoding the DNA-binding domain of a transcription activatorprotein fused to a nucleotide sequence encoding a protein of interest.The other plasmid includes a nucleotide sequence encoding thetranscription activator protein's activation domain and an unknownprotein, which may bind the protein of interest. The plasmids aretransformed into a strain of Saccharomyces cerevisiae that contains areporter gene (e.g., HIS or lacZ) whose regulatory region contains thetranscription activator's binding site. Neither of the hybrid proteinscan activate transcription of the reporter gene alone; the bait plasmidlacks an activation function, and the other plasmid cannot localize tothe transcription activator's binding sites. If the protein of interestand the unknown protein form a protein-protein complex and reconstitutethe proximity of the DNA binding domain and the activation domain, thiscomplex can bind to the regulatory region of the reporter gene, and thereporter gene will be expressed.

The two-hybrid system or related methodology can be used to screenlibraries for proteins that interact with the “bait” gene product. Byway of example, JIP-1 can be used as the bait gene product. Totalgenomic or cDNA sequences are fused to the DNA encoding an activationdomain of a transcriptional activator protein. This library and aplasmid encoding a hybrid of bait JIP-1 gene product fused to theDNA-binding domain are cotransformed into a yeast reporter strain, andthe resulting transformants are screened for those that express thereporter gene. For example, a bait JIP-1 gene sequence, such as JIP-1 ora domain of JIP-1, can be cloned into a vector such that it istranslationally fused to the DNA encoding the DNA-binding domain. of theGAL4 protein, to form a bait plasmid. Colonies are purified and thelibrary plasmids responsible for reporter gene expression are isolated.The library plasmids are subjected to DNA sequencing to determine thesequences of the gene encoding the JIP-1 binding proteins.

As an example, a cDNA library can be made by methods that are routine inthe art. This library can be inserted into vectors so that the sequencesfrom the library are fused to nucleotide sequences encoding thetranscriptional activation domain of the GAL4 protein. These vectors canthen be co-transformed, along with a bait JIP-1 gene-GAL4 fusionplasmid, into a yeast strain which contains a lacZ gene driven by apromoter that contains the GAL4 activation sequence. If any of thelibrary vectors encode hybrid proteins that bind the hybrid JIP-1protein encoded by the bait plasmid, a functional GAL4 will be formed,and the HIS3 gene will be expressed. Colonies expressing HIS3 can thenbe purified, and used to produce and isolate the JIP-1 interactingprotein using techniques routinely practiced in the art.

Therapeutic Compositions of JIP-1 Nucleic Acids, Peptides, andPolypeptides

Therapeutic compositions of the JNK inhibitor JIP-1 can be used to treatpathological conditions associated with apoptosis or transformation.These compositions can include JIP-1 polypeptides or peptides thatspecifically bind to and sequester JNK. Proteins can be purified bymethods known to those skilled in the art. Ausubel, supra. Peptides canbe synthesized by methods that are known to those skilled in the art.See, e.g., Merrifield, J. Am. Chem. Soc., 85:2149-2154 (1963).

Peptide mimetics are also included in the therapeutic compositions ofthe invention. These compounds mimic the activity of the peptide orpolypeptide, but are composed of molecules other than, or in additionto, amino acids. The design of such mimetics is described in, e.g.,Hughes, Philos. Trans. R. Soc. Lond., 290:387-394 (1980); Hodgson,Biotechnol., 9:19-21 (1991); Suckling, Sci. Prog., 75:323-359 (1991).

Therapeutic compositions of the invention also include nucleic acidsencoding a JIP-1 polypeptide or peptide. These nucleic acids can beadministered in a manner allowing their uptake and expression by cellsin vivo. Compositions containing nucleic acids can be prepared foradministration by methods that are routine in the art.

Therapeutic compositions of the invention can include one or morecompounds, e.g., nucleic acids, peptides, polypeptides, or peptidemimetics and a pharmaceutically acceptable carrier. Pharmaceuticallyacceptable carriers are biologically compatible vehicles, e.g.,physiological saline, which are suitable for administration to apatient.

Nucleic acids can be administered to the patient by standard vectorsand/or gene delivery systems. Suitable gene delivery systems includeliposomes, receptor-mediated delivery systems, naked DNA and viralvectors such as herpes viruses, retroviruses, adenoviruses andadeno-associated viruses.

Peptides can be coupled to membrane permeable peptides in order tofacilitate their uptake by cells. This can be done by colinear synthesisof a membrane permeable peptide with a peptide sequence of interest. Thetwo peptides could also be crosslinked together. Methods of coupling aredescribed in, e.g., Lin et al., J. Biol. Chem., 269:12320-12324 (1996);Rojas et al., J. Biol. Chem., 271:27456-127461 (1997). Peptides can alsobe coupled to lipids to provide membrane permeability. See, e.g.,Vijayaraghavan, J. Biol. Chem., 272:4747-4752 (1997). As an example, afatty acid can be coupled to the α-NH₂ group of the peptide as an amide.

To enable the compositions to penetrate the blood-brain barrier, theycan be delivered in encapsulated cell implants (e.g., those produced byCytoTherapeutics, Inc., Providence R.I.; see Bioworld Today 7:6 (Monday,Dec. 2, 1996)). Delivery of drugs to the brain can also be accomplishedusing RMP-7™ technology (Alkermes, Inc., Cambridge, Mass.; see BusinessWire, “Third Major Agreement for Prolease Sustained Release DrugDelivery System,” Dec. 2, 1996) or implantable wafers containing thedrug (see PR Newswire, “Implantable Wafer is First Treatment to DeliverChemotherapy Directly to Tumor Site,” Sep. 24, 1996). The compositionscan also be administered using an implantable pump for directadministration into intrathecal fluid (e.g., that made by Medtronic,Minneapolis, Minn.; see Genetic Engineering News, “NeurobiotechnologyCompanies Focus Programs on Pain and Neuroprotection,” Nov. 1, 1996).

Administration of Therapeutic Compositions

Parenteral administration, such as intravenous, subcutaneous,intramuscular, or intraperitoneal delivery routes can be used to deliverthe therapeutic compositions of the invention. Dosages for particularpatients depend upon many factors, including the patient's size, bodysurface area, age, the particular substance to be administered, time androute of administration, general health and other drugs beingadministered concurrently. The amount of therapeutic composition to beadministered to a patient can be in the range of 1 to 1000 μg/kg of bodyweights e.g., 10 to 500 μg/kg, or 20 to 200 μg/kg of body weight. Atypical dose of peptide or nucleic acid to be administered to a patientis 100 μg per kilogram of body weight.

EXAMPLES Example 1

Identification of JIP-1

The yeast two hybrid method was used to screen a mouse embryo cDNAlibrary to identify proteins that interact with JNK. The method isdescribed in detail in Fields et al., Nature, 340:245 (1989). The yeaststrain used was L40 (MATa hisΔ200 trp1-901 leu2-3,112 ade2LYS::(lexAop)₄-HIS URA3::(lexAop)₈-lacZ. Vojtek, et al., Cell, 74:205(1993). Human JNK1 fused to the LexA DNA binding domain was used as thebait. The bait plasmid, pLexA-JNK1, was constructed by blunt-endligation of a XbaI/HindIII fragment of pCMV-Flag-JNK1 (Derijard et al.,Cell, 76:1025 (1994); Sluss et al., Mol. Cell Biol., 14:8376 (1994);Gupta et al., EMBO. J., 15:2760 (1996)) into the vector pBTM116 at theSmaI and SalI sites. Yeast transformants (5.2×10⁶) were examined forgrowth on media with 25 mM 3-aminotriazole in the absence of histidine.Positive clones were confirmed by measurement of lacZ expression.

The two-hybrid screen yielded a cDNA fragment encoding a portion of aJNK binding protein. A group of 7 independent clones, corresponding tooverlapping fragments of this cDNA, was identified by sequencing.Full-length cDNA clones were obtained by screening a mouse brain λZAPIIcDNA library (Stratagene Inc.) with a random-primed cDNA fragmentcorresponding to base pairs 560-1020 of the full length cDNA. IsolatedcDNA clones were sequenced using an Applied Biosystems model 373Amachine. The sequence of the 5′ GC-rich non-coding region of the largestcDNA clone was confirmed using the Maxam-Gilbert method. Maxam et al.,Proc. Natl. Acad. Sci. USA, 74:560-564 (1977). The amino acid sequenceof the encoded protein was deduced from the cDNA sequence. Single letterabbreviations for the amino acid residues are as follows: A, Ala; C,Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M,Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; andY, Tyr.

The largest cDNA clone isolated from the mouse brain library was 2790base pairs in length, and contained a predicted coding region of 660amino acids in the same reading frame as the partial clones obtained inthe two-hybrid screen. The protein encoded by this cDNA, designated JNKinteracting protein-1 (JIP-1), contains an amino terminal JNK bindingdomain (defined by the overlapping 2-hybrid clones) and a putative SH3domain in the carboxy terminus. The structure and amino acid andnucleotide sequences of JIP-1 are shown in FIGS. 1A-1C. The JNK bindingdomain (JBD; residues 127-281, SEQ ID NO:4) and the putative SH3 domain(residues 491-540, SEQ ID NO:13) are indicated by boxes. The putativeSH3 domain of JIP-1 is highly related to the SH3 domains located in thetyrosine kinase c-fyn and the p85 subunit of PI-3′ kinase.

Human homologs of the murine JIP-1 gene can be isolated using the murineJIP-1 cDNA clones or fragments of those clones as probes, by methodsthat are routine in the art of molecular biology. For example, a libraryof human cDNA can be screened with a fragment of murine JIP-1 cDNA, andhuman cDNA hybridizing to the murine JIP-1 cDNA can be isolated, cloned,sequenced and analyzed for structural and functional similarity tomurine JIP-1. For general methods, of isolating and characterizinghomologous genes from libraries, see Sambrook et al., Molecular Cloning,A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold SpringHarbor, N.Y. (2d ed. 1989).

The tissue distribution of JIP-1 mRNA was examined by Northern blotanalysis of poly (A)⁺ RNA isolated from different murine tissues.Northern blots were performed using 2 μg of polyA⁺ RNA isolated fromvarious murine tissues (Clontech). The blots were hybridized to a probethat was prepared by labeling JIP-1 cDNA (base pairs 515-970) with[α-³²P]dCTP (Amersham International PLC) by the random priming method(Stratagene Inc.) according to the manufacturer's instructions. Theintegrity of the mRNA samples was confirmed by hybridization to an actinprobe. The blots were washed three times with 1×SSC, 0.05% SDS, and 1 mMEDTA prior to autoradiography. The results indicate that JIP-1 isexpressed in many different tissues, including brain, heart, spleen,lung, liver, muscle, kidney and testis. Highest amounts of JIP-1 mRNAwere detected in brain, kidney, and testis.

Example 2

JIP-1 Specifically Binds JNK in vivo

To test whether JIP-1 and JNK interact in vivo, co-immunoprecipitationanalysis was performed. COS-1 cells were mock-transfected or transfectedwith JIP-1 and JNK1 expression vectors. Constructs expressing epitope(HA) tagged JNK1 have been described previously. Dérijard et al., supra;Sluss et al., supra; Gupta et al., supra. Mammalian JIP-1 expressionvectors were constructed by subcloning the JIP-1 cDNA into the XbaI andHindIII sites of pCMV5 and the HindIII and EcoRI sites of pcDNA3(Invitrogen Inc.). DNA encoding the Flag epitope,-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys- (SEQ ID NO:14) (Immunex Corp.), wasinserted into JIP-1 cDNA between the DNA encoding the first two codonsof JIP-1 using insertional overlapping polymerase chain reaction (PCR).Ho et al., Gene, 77:51 (1989). Bacterial JIP-1 expression vectors wereconstructed by subcloning PCR fragments of the JIP-1 cDNA into the EcoRIand XbaI sites of pGEX-3X (Pharmacia LKB Biotechnology Inc.). GST fusionproteins were purified by affinity chromatography on GSH-agarose asdescribed previously. Smith et al., Gene, 67:31 (1988). Sequences of theconstructs were confirmed using an Applied Biosystems model 373Amachine.

For the co-immunoprecipitation experiments, transfected ormock-transfected cells were irradiated with or without UV-C (40 J/m²)and incubated for one hour. Lysates prepared from the cells wereexamined by protein immunoblot analysis using a mixture of antibodiesspecific for Flag and HA to detect Flag-JIP-1 and HA-JNK1, respectively.Cells were lysed in TLB (20 mM Tris (pH 7.5), 1% Triton X-100, 10%glycerol, 0.137 M NaCl, 25 mM sodium β-glycerophosphate, 1 mM sodiumorthovanadate, 2 mM sodium pyrophosphate, 2 mM EDTA, 10 μg/ml leupeptin,1 mM phenylmethylsulfonylfluoride). Soluble extracts were prepared bycentrifugation at 100,000×g for 30 minutes at 4° C. The extracts werepre-cleared using protein G-Sepharose (Pharmacia-LKB BiotechnologiesInc.) and incubated for one hour with monoclonal antibody to the Flagepitope (M2; IBI-Kodak) or the HA epitope (12CA5; Boehringer-Mannheim)pre-bound to protein G-Sepharose. The immunoprecipitates were washedthree times with TLB and once with 25 mM Hepes (pH 7.5), 0.2% (w/v)Triton X-100, 1 mM EDTA. Proteins were fractionated by SDS-PAGE andtransferred electrophoretically to Immobilon-P membranes (Millipore).The membranes were blocked with 10% gamma globulin-free horse serum(Gibco-BRL) and probed with the M2 monoclonal antibody, to detectFlag-JIP-1, and either the 12CA5 monoclonal antibody or a sheep anti-JNKpolyclonal antibody, to detect HA-JNK1. Immune complexes were detectedwith a second antibody coupled to horseradish peroxidase and enhancedchemiluminescence (Amersham International PLC).

JIP-1 was detected in JNK1 immunoprecipitates by protein immunoblotanalysis, and JNK1 was detected in JIP-1 immunoprecipitates.Co-immunoprecipitation of JIP-1 with JNK2, was also observed. These dataindicate that JIP-1 specifically binds JNK in vivo. Exposure to UWradiation caused no significant change in the amount of the JNK/JIP-1complex detected by co-immunoprecipitation analysis. Control experimentsperformed to examine the specificity of the interaction of JIP-1 withJNK demonstrated that the related MAP kinases ERK2 and p38 did notco-immunoprecipitate with JIP-1. The absence of co-immunoprecipitationof JIP-1 with these MAP kinases demonstrates that JIP-1 forms a specificcomplex with JNK.

This same assay can be used to determine whether a specific polypeptideis a JIP-1 polypeptide.

Example 3

JIP-1 Interacts Directly with JNK

To test whether JIP-1 interacts directly with JNK, in vitro bindingassays were performed. The putative JNK binding domain (JBD; aminoacid-residues 127-281 of JIP-1), defined by the clones obtained in thetwo-hybrid screen, was expressed as a glutathione-S-transferase (GST)fusion protein. GST-fusion proteins were purified by affinitychromatography on GSH-agarose as described previously. Smith et al.,Gene, 67:31 (1988). Recombinant JNK, prepared by in vitro translation inthe presence of [³⁵S]methionine (Dérijard et al., supra; Sluss et al.,supra; Gupta et al., supra), was incubated with GST-JIP-1 immobilized onGSH-agarose. Control experiments revealed no detectable binding of JNKisoforms to GST alone. In contrast, in assays using GST-JNK fusionproteins representing ten different human JNK isoforms, each of theseproteins exhibited similar amounts of binding to JIP-1. JIP-1 thereforeinteracts with multiple JNK isoforms.

Previous studies have demonstrated that JNK binds to the transcriptionfactors c-Jun and ATF2, and that the binding of JNK to thesetranscription factors is isoform-dependent. To test whether JIP-1binding to JNK is also isoform-dependent, a binding assay was utilized.Cell lysates (1 ml in TLB) containing JNK1, JNK2, or p38 MAP kinase,each tagged with the Flag epitope, were incubated with 5 μg GST-fusionproteins pre-bound to 10 μl glutathione-Sepharose. The GST fusionproteins used contain residues 127-202 (SEQ ID NO:15), 203-281 (SEQ IDNO:16), 164-240 (SEQ ID NO:17), or 127-281 (SEQ ID NO:4) of JIP-1. TheGST-ATF2 fusion protein contains residues 1-109 of ATF2, and the GST-Junfusion protein contains residues 1-79 of c-Jun. After incubation for onehour at 4° C. and three washes with TLB, bound proteins were detected byprotein immunoblot analysis with the M2 monoclonal antibody, which isspecific for the Flag epitope. Dérijard, et al., supra; Sluss et al.,supra; Gupta et al., supra. When ATF2 or c-jun GST fusion proteins wereused in this assay, binding to JNK1 was greater than binding to JNK2.This finding is consistent with the results of previous studies. Incontrast, when GST-JIP-1 fusion proteins containing residues 127-202 or127-281 of JIP-1 were tested in the assay, these proteins bound bothJNK1 and JNK2. The level of binding of these fusion proteins to JNK1 wassimilar to the level of binding to JNK2. The binding of JNK to JIP-1 wassignificantly greater than the binding of JNK to ATF2 or c-Jun. Controlexperiments demonstrated that JIP-1 did not bind to p38 MAP kinase.These data establish that JIP-1 binding to JNK1 is quantitativelysimilar to JIP-1 binding to JNK2, and that JNK binding to JIP-1 issignificantly greater than JNK binding to the transcription factors ATF2and c-Jun.

The GST-JIP-1 fusion proteins containing various portions of the JIP-1gene were used to define regions of JIP-1 that are required for JNKinteraction. A GST-JIP-1 fusion protein containing residues 127-281 ofJIP-1 bound both JNK1 and JNK2. No JNK binding was detected inexperiments using the central region (residues 164-240) or the carboxyterminal region (residues 203-281) of JIP-1. However, JNK bindingactivity was observed in experiments using the amino terminal region(residues 127-202 of JIP-1). These data indicate that residues 127-164of JIP-1 are required for JNK binding activity.

To more precisely define the JIP-1 sequence required for JNK bindingactivity, additional GST-JNK fusion proteins were constructed. Thesefusion proteins contained residues 135-202 (SEQ ID NO:18), 144-202 (SEQID NO:19), 154-202 (SEQ ID NO:20), 164-202 (SEQ ID NO:21), 127-143 (SEQID NO:22), 127-153 (SEQ ID NO:23), or 127-163 (SEQ ID NO:24) of JIP-1.Proteins containing residues 127-202, 135-202, 144-202, 154-202, and127-163 all bound both JNK1 and JNK2. Thus, JIP-1 residues 144-163 areimportant for the interaction of JIP-1 with JNK. As shown in FIG. 2,this region of JIP-1 shares sequence similarity with the JNK bindingdomains of ATF2 and c-Jun.

This assay can also be used to analyze a given polypeptide to determinewhether it is, or is not, a JIP-1 polypeptide.

Example 4

A Small NH₂-terminal Region of JIP-1 is Sufficient for Interaction withJNK

The effect of increasing concentrations (0, 4, 8, 16, 32, and 64 μg/ml)of a synthetic peptide corresponding to JIP-1 residues 148-174 (SEQ IDNO:3) or a control peptide having a scrambled sequence (SEQ ID NO:12) onJIP-1-JNK interaction was examined. Peptides were synthesized using anApplied Biosystems machine. Cell lysates containing Flag epitope-taggedJNK were incubated with GST-JIP-1 (residues 127-281) prebound toglutathione-Sepharose. The beads were washed, and bound proteins weredetected by protein immunoblot analysis with a Flag-specific antibody.While the control peptide had no effect, incubation with the syntheticpeptide corresponding to residues 148-174 of JIP-1 resulted in adose-dependent decrease in JIP-1 binding to JNK1, indicating that thispeptide competes with JIP-1 for binding to JNK.

The JNK binding domain of JIP-1 contains three amino acids, Lys-155,Thr-159, and Leu-160, that are conserved in the JNK binding domains ofATF2 and c-Jun (FIG. 2). A hydrophobic amino acid, Leu, is found atresidue 162 of JIP-1. ATF2 and c-Jun also contain hydrophobic aminoacids (ATF2, Phe; c-Jun, Leu) in the position corresponding to residue162 of JIP-1. To test if these conserved residues are involved in JNKbinding, the wild type JIP-1 JBD (residues 148-174) was substituted withglycines in these positions to produce the peptides shown in FIG. 3 (SEQID NOs:7-11). The mutant peptides are identical to the wild type peptideexcept for the indicated glycine substitutions. The binding of JNK1 toGST, as well as a GST fusion protein containing JIP-1 residues 127-281,was examined in the absence and presence of the synthetic peptides (64μg/ml). A peptide with a scrambled sequence (SEQ ID NO:12) was used as acontrol. The peptide representing the wild-type JIP-1 sequence caused adose-dependent inhibition of JIP-1 binding to JNK. In contrast, thecontrol peptide caused no change in JNK binding. When any of theglycine-substituted peptides was used in the assay, the inhibition ofJNK binding was greatly reduced compared to that observed with the wildtype peptide. These data indicate that Lys-155, Thr-159, Leu-160, andLeu-162 are involved in JIP-1 binding to JNK.

Example 5

JIP-1 is a Selective Inhibitor of JNK Activity

To test whether JIP-1 competes with Jun and ATF2 for interaction withJNK, the effect of JIP-1 on transcription factor-mediatedphosphorylation of exogenous substrates was analyzed using an in vitroprotein kinase assay. In these experiments, Chinese hamster ovary (CHO)cells were serum-starved for one hour. In some experiments, the cellswere treated with 10 ng/ml mouse interleukin 1 or 100 nM phorbolmyristic acetate. JNK, p38, and ERK protein kinase activity was measuredin an immune complex kinase assay using 3 μg of the substrates GST-Jun,GST-ATF2, and myelin basic protein (MBP), respectively. Protein kinaseassays were performed using 40 μl 20 mM Hepes (pH 7.4), 20 mM MgCl₂, 20mM β-glycerophosphate, 2 mM dithiothreitol, 0.1 mM sodium orthovanadate,50 μM [γ-³²P]ATP (10 Ci/mmol). After incubation for 30 minutes at 30°C., phosphorylation of substrates was analyzed by polyacrylamide gelelectrophoresis and autoradiography.

The results show that JIP-1 markedly inhibited the phosphorylation ofc-Jun by JNK. However, JIP-1 caused no significant change in thephosphorylation of substrates by the related MAP kinases p38 and ERK2.JIP-1 is thus a selective inhibitor of JNK.

This same assay can be used to determine whether a specific polypeptidehas the same phosphorylation function of wildtype JIP-1.

Example 6

Expression of JIP-1 Inhibits Targets of the JNK-regulated SignalTransduction Pathway

The effect of JIP-1 on targets of the JNK signal transduction pathway,including the transcription factors c-Jun, ATF2 and Elk-1, was examinedto determine whether JIP-1 inhibits signal transduction by JNK.

In these experiments, CHO cells were cotransfected with constructsencoding the transcription factors and JIP-1 using previously describedmethods. Dérijard et al., supra; Sluss et al., supra; Gupta et al.,supra. A luciferase reporter plasmid was used to monitor the expressionof the transcription factors. Transfection efficiency was determinedusing a β-galactosidase expression vector. Constructs used in theseexperiments included GAL4 fusions with the c-myc, Sp1 and VP16activation domains (described in Whitmarsh et al., Science, 269:403(1995); Davis, Science, 269:403 (1995); Gille et al., Curr. Biol.,5:1191 (1995)). Other constructs used were GAL4-c-Jun, in which GAL4 isfused to c-Jun; GAL4-c-Jun (S63A/S73A), in which GAL4 is fused to amutant c-Jun in which the serines at positions 63 and 73 have beenchanged to alanines; GAL4-ATF2, in which GAL4 is fused to wild type ATF2(ATF2(Thr-69,71)); GAL4-ATF2 (T69A/T71A) (ATF2(Ala-69/71)), in whichGAL4 has been fused to a mutant ATF2 in which the tyrosines at positions69 and 71 have been changed to alanines; GAL4-Elk-1, in which GAL4 hasbeen fused to Elk-1; and GAL4-Elk-1 (S383A), in which GAL4 has beenfused to a mutant Elk-1 in which the serine at position 383 has beenchanged to an alanine. Gupta et al., EMBO J, 15:2760-2770 (1996);Raingeaud et al., Mol. Cell. Biol., 16:1247-1255 (1996); Whitmarsh etal., Science, 269:403-407 (1995).

Insertional overlapping PCR, described in detail in Ho, supra, was usedto construct expression vectors for the JNK binding domain (JBD; aminoacid residues 127-281) of JIP-1 and for a mutant JIP-1 lacking the SH3(amino acid residues 491-540) domain.

Transfected cells were activated by treatment with 10% (v/v) fetal calfserum, and luciferase and β-galactosidase activities were measured incell lysates at 48 hours post-transfection. The results are shown inFIGS. 4A-4C. The data are presented as the ratio of luciferase activity(light units) to β-galactosidase activity (OD units) measured in thecell extracts (mean±SEM (n=3)), and are normalized to the luciferaseactivity detected in the absence of JBD (FIGS. 4A and 4B) or ATF2 (FIG.4C).

Control experiments demonstrated that the JNK binding domain (JBD;residues 127-281 of JIP-1) did not inhibit reporter gene expressionmediated by the activation domains of c-Myc, E1a, Sp1, and VP16 (FIG.4A). Significant inhibition of c-Jun and ATF2 transcriptional activityby JIP-1 was observed, however (FIG. 4B). The partial inhibition ofElk-1 transcriptional activity shown in FIG. 4B may reflect anassociation of both ERK and JNK MAP kinases with Elk-1 regulation.Mutation of the JNK phosphorylation sites in ATF2, c-Jun, and Elk-1caused lower basal transcriptional activity that was not markedlyinhibited by JIP-1.

As shown in FIG. 4C, inhibition of JNK-regulated gene expression wasobserved in experiments using wild-type JIP-1, the JNK binding domain(JBP), and ASH3, a JIP-1 deletion mutant lacking the SH3 domain.Together, these data indicate that JIP-1 suppresses JNK-regulated geneexpression, and that the JNK binding domain is sufficient for thisactivity.

This assay can be used to determine whether specific polypeptides havethe same effect on signal transduction as full length, wildtype JIP-1.

Example 7

Subcellular Distribution of JIP-1

The subcellular distribution of JIP-1 was analyzed by performingindirect immunofluorescence on JIP-1-expressing cells. In theseexperiments, the cells were grown on coverslips, fixed with 4%paraformaldehyde, permeabilized with 0.25% Triton X-100, and processedfor immunofluorescence microscopy. JIP-1 was detected in the cytoplasm,but not the nucleus, of control and UV-irradiated cells. In contrast,JNK is detected in both the cytoplasmic and nuclear compartments.

It is likely that it is JNK in the nucleus which is involved in theregulation of gene expression. Since JIP-1 is cytoplasmic, it wasunclear how it could inhibit the nuclear function of JNK. To investigatethe mechanism of JIP-1 action, the distribution of JNK was examined incells that had been transfected with either hemagglutinin (HA)-taggedJNK1 (HA-JNK1) alone, or HA-JNK1 and Flag-tagged JIP-1 (Flag-JIP-1). Inthese experiments, the cells were exposed to a potent JNK activator (40J/² UV-C) for one hour prior to processing for immunofluorescence. Theprimary antibodies used were rabbit anti-HA (12CA5; BoehringerMannheim), which recognizes HA-tagged JNK1; and mouse monoclonalanti-Flag (M2; IBI-Kodak), which recognizes Flag-tagged JIP-1. Thesecondary antibodies were Texas Red-goat anti-mouse Ig and fluorosceinisothiocyanate-conjugated donkey anti-rabbit Ig (JacksonImmunoresearch). Procedures for digital imaging microscopy and imagerestoration using the exhaustive photon reassignment algorithm aredescribed in Carrington et al., Science, 268:1483 (1995). Individualoptical sections were inspected using computer graphics software on aSilicon Graphics workstation.

The results demonstrate that expression of JIP-1 reduces the amount ofnuclear JNK detected in control and UV-irradiated cells. In contrast,JIP-1 expression has no significant effect on the subcellulardistribution of p38 MAP kinase, which is also located in both nuclearand cytoplasmic compartments of cultured cells. These data indicate thatJIP-1 expression results in selective cytoplasmic retention of JNK. Itis likely that this cytoplasmic retention contributes to the ability ofJIP-1 to inhibit JNK-mediated signal transduction.

Example 8

JIP-1 Inhibits Nerve Growth Factor Withdrawal-Induced Apoptosis

The effect of JIP-1 on this biological response was investigated todetermine whether JIP-1 inhibits the biological effects of the JNKsignaling pathway. NGF withdrawal-induced apoptosis of differentiatedPC12 cells was examined following transfection with the expressionvectors pCDNA3 (control) and pCDNA3-Flag-JIP-1 (containing residues127-281 of JIP-1) using methods described previously. Xia et al.,Science, 270:1326 (1995). The percentage of apoptotic cells in the totaltransfected cell population was blind-scored and quantitated. This assayscores adherent cells with an apoptotic morphology at 17 hours followingNGF withdrawal. Cells that have completed apoptosis are nonadherent andare not scored. Thus, while the cumulative extent of apoptosis is large(100%), lower numbers of apoptotic cells are detected at a single timepoint.

The results are shown in FIG. 5. The data shown are representative ofthree independent experiments. The error bars in the graph indicate theSEM and the numbers within each bar are the total number of transfectedcells counted. Expression of the JNK binding domain of JIP-1 (JBD;residues 127-281) in differentiated PC12 cells markedly reducedapoptosis following NGF-withdrawal. These data demonstrate that JIP-1suppresses JNK-mediated signal transduction. This assay can be used totest new JIP-1 polypeptides.

Example 9

JIP-1 Inhibits Pre-B Cell Transformation by BCR-ABL

The BCR-ABL oncogene, which is associated with chronic myelogenousleukemia (CML), causes JNK activation in the absence of increased ERKactivity. Raitano et al., Proc. Natl. Acad. Sci. USA, 92:11746 (1995).Oncogenic transformation by BCR-ABL may be mediated in part by the JNKsignaling pathway. Since JIP-1 can inhibit JNK activity, the effect ofJIP-1 expression on oncogenic transformation was examined. Plasmidvectors expressing v-ABL or BCR-ABL and Flag-tagged JBD (residues127-281) of JIP-1 were used to transfect 293 cells. JNK activity wasthen measured in an immune complex kinase assay of lysates of thetransfected 293 cells, using a polyclonal JNK antibody and GST-Jun asthe substrate. Control experiments demonstrated that v-ABL and BCR-ABLcaused constitutive JNK activation (approximately 5-fold), which wasblocked by co-expression of the JBD of JIP-1. JIP-1 can thereforeinhibit BCR-ABL-mediated activation of JNK.

To examine the effect of JIP-1 on BCR-ABL-mediated cellulartransformation, bone marrow transformation assays were performed usingrecombinant retroviruses packaged with 293T cells as described inSawyers et al., J. Exp. Med., 181:307 (1995). Bicistronic retrovirusesexpressing BCR-ABL, alone, or in combination with the Flag-tagged JBD(residues 127-281) of JIP-1, were prepared by subcloning p185BCR-ABLinto the ClaI site of pSRαTK, downstream of the internal TK promoter, tocreate pSRαMSVTKp185. The JBD was subcloned into the upstream EcoRI sitein the sense and antisense orientations. The structures of theretroviral constructs are shown in FIG. 6.

Transfection of 293 cells with these retroviral constructs resulted inexpression of the appropriate proteins, as demonstrated by immunoblotanalysis of whole cell lysates using antibodies specific for ABL todetect BCR-ABL, and antibodies to Flag to detect the JBD of JIP-1. Therecombinant retroviruses were then used to infect primary mouse marrowcells, and the transformation of pre-B cells was monitored in culture.FIG. 6 shows the mean density (±SE) of non-adherent pre-B cells on day10 post infection. The data shown are derived from three independentexperiments plated in triplicate. As expected, BCR-ABL caused pre-B celltransformation. The JBD of JIP-1 caused marked inhibition oftransformation when present in the sense, but not the anti-sense,orientation. In some cultures infected with BCR-ABL and JBD, pre-B celloutgrowths were detected after 3-4 weeks, but these clones demonstratedno expression of JBD by protein immunoblot analysis. Since JIP-1inhibits JNK signaling, these results indicate that the JNK pathway isrequired for pre-B cell transformation by BCR-ABL.

The demonstration that JNK is involved in both apoptosis and oncogenictransformation indicates that the biological actions of the JNK signaltransduction pathway depend on the specific cellular context. Theintegration of JNK with other signal transduction pathways may be animportant determinant of the functional consequences of JNK activation.The ability of JIP-1, an inhibitor of the JNK signal transductionpathway, to block both transformation and apoptosis is consistent withthis hypothesis.

The physiological function of JIP-1 may be to suppress signaltransduction by the JNK pathway. For example, JIP-1 may compete withsubstrates that bind JNK. Alternatively, JIP-1 may have a more directrole in targeting JNK to specific regions of the cell or to specificsubstrates. Since JIP-1 causes redistribution of JNK within the cell,JIP-1 may function as a cytoplasmic anchor for JNK. The tethering of JNKin the cytoplasm by interactions with JIP-1 provides a mechanism forcontrolling signal transduction by the JNK pathway, and the relatedphenomena of apoptosis and transformation.

This assay can be used to determine if specific polypeptides have thesame effect on cellular transformation as full length, wildtype JIP-1.

Example 10

Screening for Peptides with JIP-1 Activity

Peptides suspected of having JIP-1 activity can be tested in the JNKbinding assay described supra. Peptides are synthesized by methods thatare well known to those skilled in the art; for example, using anApplied Biosystems synthesizer. Cell lysates containing Flagepitope-tagged JNK are incubated with GST-JIP-1 (residues 127-281) boundto glutathione-Sepharose, either with or without synthetic peptides (0,4, 8, 16, 32 or 64 μg/ml), for one hour at 4° C. The beads are washed inTLB, and the bound proteins are detected by protein immunoblot analysiswith a Flag-specific antibody, e.g., M2. Synthetic peptides with JIP-1activity are those which inhibit the interaction of JIP-1 with JNK, asdetected in this assay. These synthetic peptides should possess at least60% of the binding activity of JIP-1.

Example 11

Therapeutic Applications

JIP-1 is shown herein to be capable of inhibiting apoptosis andtransformation. Compositions containing JIP-1 polypeptides or nucleicacids can be administered to treat conditions characterized by thesephenomena. Nucleic acids can be administered by, methods described in,e.g., Ausubel, et al., supra. A standard dosage would be from 1 to 1000μg/kg of body weight. Polypeptides, peptides and peptide mimetics of theinvention can be formulated according to procedures which are well knownto those skilled in the art. A standard dosage of polypeptide also wouldbe from 1 to 1000, e.g., 10 to 500, or 20 to 200 μg/kg of body weight.

Animal models for testing the effect of JIP-1 therapeutic compositionsinclude the bcr-abl leukemia model. Daley et al., Science, 247:824-830(1990). Other animal models that can be used include the myc/rastransformation model. Sinn et al., Cell, 49:465-475 (1987). Animalmodels for testing the effect of JIP-1 therapeutic compositions onconditions associated with apoptosis include a model of excitotoxicstress in the hippocampus, and a model of E2F-1 induced apoptosis.Ben-Ari et al., Neuroscience, 14:375-403 (1985); Field et al., Cell,85:549-561 (1996). Other conditions that can be treated with JIP-1compositions include liver damage (Mendelson et al., Proc. Natl. Acad.Sci. USA, 93:12908-12913 (1996)); kidney disease and organtransplantation (DeMari et al., Am. J. Physiol., 272:F292-F298 (1997));and heart disease (Pombo et al., J. Biol. Chem., 269:26546-26551 (1994);Force et al., Circ. Res., 78:947-953 (1996).

Example 12

Diagnostic Applications

The polypeptides and antibodies of the invention can be used to detector monitor JIP-1 expression. Levels of JIP-1 protein in a sample can beassayed by any standard technique. For example, JIP-1 protein expressioncan be monitored by standard immunological or immunohistochemicalprocedures using the antibodies described herein. See, e.g., Ausubel etal., supra; Bancroft et al., Theory and Practice of HistologicalTechniques, Churchill Livingstone (1982). Alternatively, JIP-1expression can be assayed by standard Northern blot analysis, or can beaided by PCR. See Ausubel, supra; Ehrlich, ed., PCR Technology:Principles and Applications for DNA Amplification, Stockton Press, NewYork. Point mutations in the JIP-1 sequence can be detected using wellknown nucleic acid mismatch detection techniques. Lower than normallevels of JIP-1 would have the effect of altering apoptosis, whilehigher than normal levels would cause immune suppression, alteredinflammatory responses, and alterations in tumor growth.

Other Embodiments

The invention also includes naturally occurring andnon-naturally-occurring allelic variants of JIP-1. Compared to the mostcommon naturally occurring nucleotide sequence encoding JIP-1, thenucleic acid sequence encoding allelic variants may have a substitution,deletion, or addition of one or more nucleotides. The preferred allelicvariants are functionally equivalent to naturally occurring JIP-1.

It is to be understood that while the invention has been described inconjunction with the detailed description thereof, that the foregoingdescription is intended to illustrate and not limit the scope of theinvention, which is defined by the scope of the appended claims. Otheraspects, advantages and modifications are within the scope of thefollowing claims.

What is claimed is:
 1. A substantially pure JNK-interacting protein 1 (JIP-1) polypeptide, the amino acid sequence of which comprises SEQ ID NO:1, or SEQ ID NO:1 with at least one conservative amino acid substitution, wherein the polypeptide specifically inhibits the activity of c-Jun NH₂ terminal kinase (JNK).
 2. A substantially pure polypeptide, the amino acid sequence of which comprises SEQ ID NO:4, or SEQ ID NO:4 with at least one conservative amino acid substitution, wherein the polypeptide specifically inhibits the activity of c-Jun NH₂ terminal kinase (JNK).
 3. The polypeptide of claim 2, wherein the polypeptide is modified by attachment of a hydrophobic moiety.
 4. The polypeptide of claim 2, wherein the polypeptide is modified by attachment of a peptide that facilitates uptake of the polypeptide by a cell.
 5. The polypeptide of claim 1, wherein the polypeptide is modified by attachment of a hydrophobic moiety.
 6. The polypeptide of claim 1, wherein the polypeptide is modified by attachment of a peptide that facilitates uptake of the polypeptide by a cell.
 7. A substantially pure polypeptide, the amino acid sequence of which comprises SEQ ID NO:3, or SEQ ID NO:3 with at least one conservative amino acid substitution, wherein the polypeptide specifically inhibits the activity of c-Jun NH₂ terminal kinase (JNK).
 8. The polypeptide of claim 7, wherein the polypeptide is modified by attachment of a hydrophobic moiety.
 9. The polypeptide of claim 7, wherein the polypeptide is modified by attachment of a peptide that facilitates uptake of the polypeptide by a cell.
 10. A substantially pure polypeptide, the amino acid sequence of which comprises any one of SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, or SEQ ID NO:24; or SEQ ID NO:15, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, or SEQ ID NO:24 with at least one conservative amino acid substitution, wherein the polypeptide specifically inhibits the activity of c-Jun NH₂ terminal kinase (JNK).
 11. The polypeptide of claim 10, wherein the polypeptide is modified by attachment of a hydrophobic moiety.
 12. The polypeptide of claim 10, wherein the polypeptide is modified by attachment of a peptide that facilitates uptake of the polypeptide by a cell.
 13. A substantially pure polypeptide, the amino acid sequence of which comprises any one of SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:21, or SEQ ID NO:22; or SEQ ID NO:13, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:21, or SEQ ID NO:22 with at least one conservative amino acid substitution.
 14. The polypeptide of claim 13, wherein the polypeptide is modified by attachment of a hydrophobic moiety.
 15. The polypeptide of claim 13, wherein the polypeptide is modified by attachment of a peptide that facilitates uptake of the polypeptide by a cell. 